Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition

Lydia C. Garas, Cristiano Feltrin, M. Kristina Hamilton, Jill V. Hagey, James D. Murray, Luciana R. Bertolini, Marcelo Bertolini, Helen E Raybould, Elizabeth A. Maga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Malnutrition remains a leading contributor to the morbidity and mortality of children under the age of five worldwide. However, the underlying mechanisms are not well understood necessitating an appropriate animal model to answer fundamental questions and conduct translational research into optimal interventions. One potential intervention is milk from livestock that more closely mimics human milk by increased levels of bioactive components that can promote a healthy intestinal epithelium. We tested the ability of cow milk and milk from transgenic cows expressing human lactoferrin at levels found in human milk (hLF milk) to mitigate the effects of malnutrition at the level of the intestine in a pig model of malnutrition. Weaned pigs (3 weeks old) were fed a protein and calorie restricted diet for five weeks, receiving cow, hLF or no milk supplementation daily from weeks 3-5. After three weeks, the restricted diet induced changes in growth, blood chemistry and intestinal structure including villous atrophy, increased ex vivo permeability and decreased expression of tight junction proteins. Addition of both cow and hLF milk to the diet increased growth rate and calcium and glucose levels while promoting growth of the intestinal epithelium. In the jejunum hLF milk restored intestinal morphology, reduced permeability and increased expression of anti-inflammatory IL-10. Overall, this pig model of malnutrition mimics salient aspects of the human condition and demonstrates that cow milk can stimulate the repair of damage to the intestinal epithelium caused by protein and calorie restriction with hLF milk improving this recovery to a greater extent.

Original languageEnglish (US)
Pages (from-to)665-678
Number of pages14
JournalFood and Function
Volume7
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Lactoferrin
lactoferrin
Malnutrition
malnutrition
Milk
Swine
milk
swine
Intestinal Mucosa
intestinal mucosa
Human Milk
breast milk
cows
Permeability
permeability
Growth
Diet
Tight Junction Proteins
Child Mortality
Protein-Restricted Diet

ASJC Scopus subject areas

  • Food Science

Cite this

Garas, L. C., Feltrin, C., Kristina Hamilton, M., Hagey, J. V., Murray, J. D., Bertolini, L. R., ... Maga, E. A. (2016). Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition. Food and Function, 7(2), 665-678. https://doi.org/10.1039/c5fo01217a

Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition. / Garas, Lydia C.; Feltrin, Cristiano; Kristina Hamilton, M.; Hagey, Jill V.; Murray, James D.; Bertolini, Luciana R.; Bertolini, Marcelo; Raybould, Helen E; Maga, Elizabeth A.

In: Food and Function, Vol. 7, No. 2, 01.02.2016, p. 665-678.

Research output: Contribution to journalArticle

Garas, LC, Feltrin, C, Kristina Hamilton, M, Hagey, JV, Murray, JD, Bertolini, LR, Bertolini, M, Raybould, HE & Maga, EA 2016, 'Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition', Food and Function, vol. 7, no. 2, pp. 665-678. https://doi.org/10.1039/c5fo01217a
Garas LC, Feltrin C, Kristina Hamilton M, Hagey JV, Murray JD, Bertolini LR et al. Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition. Food and Function. 2016 Feb 1;7(2):665-678. https://doi.org/10.1039/c5fo01217a
Garas, Lydia C. ; Feltrin, Cristiano ; Kristina Hamilton, M. ; Hagey, Jill V. ; Murray, James D. ; Bertolini, Luciana R. ; Bertolini, Marcelo ; Raybould, Helen E ; Maga, Elizabeth A. / Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition. In: Food and Function. 2016 ; Vol. 7, No. 2. pp. 665-678.
@article{d36f1504ec9f4d328e7cc30275edf80e,
title = "Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition",
abstract = "Malnutrition remains a leading contributor to the morbidity and mortality of children under the age of five worldwide. However, the underlying mechanisms are not well understood necessitating an appropriate animal model to answer fundamental questions and conduct translational research into optimal interventions. One potential intervention is milk from livestock that more closely mimics human milk by increased levels of bioactive components that can promote a healthy intestinal epithelium. We tested the ability of cow milk and milk from transgenic cows expressing human lactoferrin at levels found in human milk (hLF milk) to mitigate the effects of malnutrition at the level of the intestine in a pig model of malnutrition. Weaned pigs (3 weeks old) were fed a protein and calorie restricted diet for five weeks, receiving cow, hLF or no milk supplementation daily from weeks 3-5. After three weeks, the restricted diet induced changes in growth, blood chemistry and intestinal structure including villous atrophy, increased ex vivo permeability and decreased expression of tight junction proteins. Addition of both cow and hLF milk to the diet increased growth rate and calcium and glucose levels while promoting growth of the intestinal epithelium. In the jejunum hLF milk restored intestinal morphology, reduced permeability and increased expression of anti-inflammatory IL-10. Overall, this pig model of malnutrition mimics salient aspects of the human condition and demonstrates that cow milk can stimulate the repair of damage to the intestinal epithelium caused by protein and calorie restriction with hLF milk improving this recovery to a greater extent.",
author = "Garas, {Lydia C.} and Cristiano Feltrin and {Kristina Hamilton}, M. and Hagey, {Jill V.} and Murray, {James D.} and Bertolini, {Luciana R.} and Marcelo Bertolini and Raybould, {Helen E} and Maga, {Elizabeth A.}",
year = "2016",
month = "2",
day = "1",
doi = "10.1039/c5fo01217a",
language = "English (US)",
volume = "7",
pages = "665--678",
journal = "Food and Function",
issn = "2042-6496",
publisher = "Royal Society of Chemistry",
number = "2",

}

TY - JOUR

T1 - Milk with and without lactoferrin can influence intestinal damage in a pig model of malnutrition

AU - Garas, Lydia C.

AU - Feltrin, Cristiano

AU - Kristina Hamilton, M.

AU - Hagey, Jill V.

AU - Murray, James D.

AU - Bertolini, Luciana R.

AU - Bertolini, Marcelo

AU - Raybould, Helen E

AU - Maga, Elizabeth A.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Malnutrition remains a leading contributor to the morbidity and mortality of children under the age of five worldwide. However, the underlying mechanisms are not well understood necessitating an appropriate animal model to answer fundamental questions and conduct translational research into optimal interventions. One potential intervention is milk from livestock that more closely mimics human milk by increased levels of bioactive components that can promote a healthy intestinal epithelium. We tested the ability of cow milk and milk from transgenic cows expressing human lactoferrin at levels found in human milk (hLF milk) to mitigate the effects of malnutrition at the level of the intestine in a pig model of malnutrition. Weaned pigs (3 weeks old) were fed a protein and calorie restricted diet for five weeks, receiving cow, hLF or no milk supplementation daily from weeks 3-5. After three weeks, the restricted diet induced changes in growth, blood chemistry and intestinal structure including villous atrophy, increased ex vivo permeability and decreased expression of tight junction proteins. Addition of both cow and hLF milk to the diet increased growth rate and calcium and glucose levels while promoting growth of the intestinal epithelium. In the jejunum hLF milk restored intestinal morphology, reduced permeability and increased expression of anti-inflammatory IL-10. Overall, this pig model of malnutrition mimics salient aspects of the human condition and demonstrates that cow milk can stimulate the repair of damage to the intestinal epithelium caused by protein and calorie restriction with hLF milk improving this recovery to a greater extent.

AB - Malnutrition remains a leading contributor to the morbidity and mortality of children under the age of five worldwide. However, the underlying mechanisms are not well understood necessitating an appropriate animal model to answer fundamental questions and conduct translational research into optimal interventions. One potential intervention is milk from livestock that more closely mimics human milk by increased levels of bioactive components that can promote a healthy intestinal epithelium. We tested the ability of cow milk and milk from transgenic cows expressing human lactoferrin at levels found in human milk (hLF milk) to mitigate the effects of malnutrition at the level of the intestine in a pig model of malnutrition. Weaned pigs (3 weeks old) were fed a protein and calorie restricted diet for five weeks, receiving cow, hLF or no milk supplementation daily from weeks 3-5. After three weeks, the restricted diet induced changes in growth, blood chemistry and intestinal structure including villous atrophy, increased ex vivo permeability and decreased expression of tight junction proteins. Addition of both cow and hLF milk to the diet increased growth rate and calcium and glucose levels while promoting growth of the intestinal epithelium. In the jejunum hLF milk restored intestinal morphology, reduced permeability and increased expression of anti-inflammatory IL-10. Overall, this pig model of malnutrition mimics salient aspects of the human condition and demonstrates that cow milk can stimulate the repair of damage to the intestinal epithelium caused by protein and calorie restriction with hLF milk improving this recovery to a greater extent.

UR - http://www.scopus.com/inward/record.url?scp=84959419826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959419826&partnerID=8YFLogxK

U2 - 10.1039/c5fo01217a

DO - 10.1039/c5fo01217a

M3 - Article

VL - 7

SP - 665

EP - 678

JO - Food and Function

JF - Food and Function

SN - 2042-6496

IS - 2

ER -