Milk-borne epidermal growth factor modulates intestinal transforming growth factor-α levels in neonatal rats

Bohuslav Dvorak, Catherine S. Williams, Debra L. McWilliam, Hisae Shinohara, Jessica A. Dominguez, Robert S. McCuskey, Anthony F Philipps, Otakar Koldovsky

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) is present in milk from various mammalian species, but its physiologic function in neonatal development remains unclear. Transforming growth factor-α (TGF-α) is a peptide structurally related to EGF, and its presence is detected in the developing small intestine of rats. The purpose of the present study was to examine the effect of milk-borne EGF on endogenous production of EGF and TGF-α in the small intestine of suckling rats. Neonatal rats were fed via gastrostomy either growth factor-free rat milk substitute (RMS) or RMS supplemented with EGF (100 ng/mL of RMS) from 8 to 12 d of age. Artificially reared rats were then compared with their dam-fed littermates. Animals fed the EGF-deficient diet RMS had markedly increased EGF and TGF-α mRNA levels in duodenum and ileum compared with dam-fed controls and significantly elevated total intestinal content of TGF-α peptide. Intestinal EGF content and EGF serum levels were significantly decreased in the RMS group compared with controls. The addition of EGF to the RMS diet normalized TGF-α mRNA levels in the duodenum and ileum, EGF mRNA levels in the ileum, and total intestinal TGF-α content and EGF serum levels to the levels measured in dam-fed littermates. Motility studies showed that enteral administration of EGF did not affect stomach emptying and intestinal transit. These studies indicate that exogenous milk- borne EGF modulates endogenous production of TGF-α in developing small intestine. It is likely that neither TGF-α nor EGF are solely responsible for small intestinal overgrowth of artificially reared neonatal rats.

Original languageEnglish (US)
Pages (from-to)194-200
Number of pages7
JournalPediatric Research
Volume47
Issue number2
StatePublished - Feb 2000
Externally publishedYes

Fingerprint

Transforming Growth Factors
Epidermal Growth Factor
Milk
Milk Substitutes
Small Intestine
Ileum
Duodenum
Messenger RNA
Diet
Peptides
Gastrointestinal Contents
Gastrostomy
Serum
Intercellular Signaling Peptides and Proteins
Stomach

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Dvorak, B., Williams, C. S., McWilliam, D. L., Shinohara, H., Dominguez, J. A., McCuskey, R. S., ... Koldovsky, O. (2000). Milk-borne epidermal growth factor modulates intestinal transforming growth factor-α levels in neonatal rats. Pediatric Research, 47(2), 194-200.

Milk-borne epidermal growth factor modulates intestinal transforming growth factor-α levels in neonatal rats. / Dvorak, Bohuslav; Williams, Catherine S.; McWilliam, Debra L.; Shinohara, Hisae; Dominguez, Jessica A.; McCuskey, Robert S.; Philipps, Anthony F; Koldovsky, Otakar.

In: Pediatric Research, Vol. 47, No. 2, 02.2000, p. 194-200.

Research output: Contribution to journalArticle

Dvorak, B, Williams, CS, McWilliam, DL, Shinohara, H, Dominguez, JA, McCuskey, RS, Philipps, AF & Koldovsky, O 2000, 'Milk-borne epidermal growth factor modulates intestinal transforming growth factor-α levels in neonatal rats', Pediatric Research, vol. 47, no. 2, pp. 194-200.
Dvorak B, Williams CS, McWilliam DL, Shinohara H, Dominguez JA, McCuskey RS et al. Milk-borne epidermal growth factor modulates intestinal transforming growth factor-α levels in neonatal rats. Pediatric Research. 2000 Feb;47(2):194-200.
Dvorak, Bohuslav ; Williams, Catherine S. ; McWilliam, Debra L. ; Shinohara, Hisae ; Dominguez, Jessica A. ; McCuskey, Robert S. ; Philipps, Anthony F ; Koldovsky, Otakar. / Milk-borne epidermal growth factor modulates intestinal transforming growth factor-α levels in neonatal rats. In: Pediatric Research. 2000 ; Vol. 47, No. 2. pp. 194-200.
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