Migration and proliferation characteristics of SV-40 transformed human corneal epithelial cells in response to neuropeptides

Purpose. Neuropeptides have been postulated to have a major role in wound healing and previously have been shown to affect migration and proliferation of corneal epithelial cells. We set out to partially characterize the properties of a unique immortal cell line derived from human corneal epithelial cells. Methods. Effects of serum, EGF, VIP and Substance P on migration of SV 40 transformed, human corneal epithelial cells (Araki-Sasaki, et al, IOVS 36:614-621, 1995), were determined using a modified checkerboard analysis in 96-well migration chambers. Proliferation characteristics of these cells in response to the same factors were determined using a DNA fluorometric assay with Hoechst 33258. Results. Our results are consistent with previously reported results in primary human epithelial cell culture. Additionally, the positive effect of neuropeptides on migration and proliferation have been documented. Conclusion. These results further characterize this unique cell line and suggest the significant role of neuropeptides in epithelial cell proliferation and migration.