Microvascular solute and water transport

Fitz Roy E Curry

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Objective: This review evaluates (1) the regulation of water and solute transport across the endothelial barrier in terms of pore theory and the glycocalyx-junction-break model of capillary permeability; and (2) the mechanisms regulating permeability based on experiments using cultured endothelial cells and intact microvessels. Conclusions: The current form of the glycocalyx-junction-break model of capillary permeability describes the selectivity of the capillary wall (pore size) in terms of the space between the fibers of a quasi-periodic matrix on the endothelial cell surface, and the area for exchange (pore number) in terms of the length and frequency of breaks in the tight junction strands. An independent test of this model in a range of mammalian microvascular beds is new experimental evidence that the colloid osmotic pressure of plasma proteins is developed across the glycocalyx, not across the whole microvessel wall. We are beginning to understand that endothelial cells may change their phenotype in response to physical and chemical stresses. Such changes in phenotype may explain changes in the regulation of endothelial barrier function in intact microvessels that have previously been exposed to injury and differences in the regulation of contractile mechanisms between endothelial cells in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)17-31
Number of pages15
JournalMicrocirculation
Volume12
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Glycocalyx
Endothelial Cells
Microvessels
Water
Capillary Permeability
Phenotype
Tight Junctions
Osmotic Pressure
Colloids
Blood Proteins
Cultured Cells
Permeability
Wounds and Injuries

Keywords

  • Capillary permeability
  • Endothelial barrier
  • Endothelial claudins
  • Endothelial contraction
  • Endothelial glycocalyx
  • Pore theory
  • Rac-1
  • Small GTP ases
  • Thrombin
  • Tight junction
  • VE-cadherin

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine

Cite this

Microvascular solute and water transport. / Curry, Fitz Roy E.

In: Microcirculation, Vol. 12, No. 1, 01.2005, p. 17-31.

Research output: Contribution to journalArticle

Curry, Fitz Roy E. / Microvascular solute and water transport. In: Microcirculation. 2005 ; Vol. 12, No. 1. pp. 17-31.
@article{e884f8fba5104722919c550bab34a946,
title = "Microvascular solute and water transport",
abstract = "Objective: This review evaluates (1) the regulation of water and solute transport across the endothelial barrier in terms of pore theory and the glycocalyx-junction-break model of capillary permeability; and (2) the mechanisms regulating permeability based on experiments using cultured endothelial cells and intact microvessels. Conclusions: The current form of the glycocalyx-junction-break model of capillary permeability describes the selectivity of the capillary wall (pore size) in terms of the space between the fibers of a quasi-periodic matrix on the endothelial cell surface, and the area for exchange (pore number) in terms of the length and frequency of breaks in the tight junction strands. An independent test of this model in a range of mammalian microvascular beds is new experimental evidence that the colloid osmotic pressure of plasma proteins is developed across the glycocalyx, not across the whole microvessel wall. We are beginning to understand that endothelial cells may change their phenotype in response to physical and chemical stresses. Such changes in phenotype may explain changes in the regulation of endothelial barrier function in intact microvessels that have previously been exposed to injury and differences in the regulation of contractile mechanisms between endothelial cells in vivo and in vitro.",
keywords = "Capillary permeability, Endothelial barrier, Endothelial claudins, Endothelial contraction, Endothelial glycocalyx, Pore theory, Rac-1, Small GTP ases, Thrombin, Tight junction, VE-cadherin",
author = "Curry, {Fitz Roy E}",
year = "2005",
month = "1",
doi = "10.1080/10739680590894993",
language = "English (US)",
volume = "12",
pages = "17--31",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Microvascular solute and water transport

AU - Curry, Fitz Roy E

PY - 2005/1

Y1 - 2005/1

N2 - Objective: This review evaluates (1) the regulation of water and solute transport across the endothelial barrier in terms of pore theory and the glycocalyx-junction-break model of capillary permeability; and (2) the mechanisms regulating permeability based on experiments using cultured endothelial cells and intact microvessels. Conclusions: The current form of the glycocalyx-junction-break model of capillary permeability describes the selectivity of the capillary wall (pore size) in terms of the space between the fibers of a quasi-periodic matrix on the endothelial cell surface, and the area for exchange (pore number) in terms of the length and frequency of breaks in the tight junction strands. An independent test of this model in a range of mammalian microvascular beds is new experimental evidence that the colloid osmotic pressure of plasma proteins is developed across the glycocalyx, not across the whole microvessel wall. We are beginning to understand that endothelial cells may change their phenotype in response to physical and chemical stresses. Such changes in phenotype may explain changes in the regulation of endothelial barrier function in intact microvessels that have previously been exposed to injury and differences in the regulation of contractile mechanisms between endothelial cells in vivo and in vitro.

AB - Objective: This review evaluates (1) the regulation of water and solute transport across the endothelial barrier in terms of pore theory and the glycocalyx-junction-break model of capillary permeability; and (2) the mechanisms regulating permeability based on experiments using cultured endothelial cells and intact microvessels. Conclusions: The current form of the glycocalyx-junction-break model of capillary permeability describes the selectivity of the capillary wall (pore size) in terms of the space between the fibers of a quasi-periodic matrix on the endothelial cell surface, and the area for exchange (pore number) in terms of the length and frequency of breaks in the tight junction strands. An independent test of this model in a range of mammalian microvascular beds is new experimental evidence that the colloid osmotic pressure of plasma proteins is developed across the glycocalyx, not across the whole microvessel wall. We are beginning to understand that endothelial cells may change their phenotype in response to physical and chemical stresses. Such changes in phenotype may explain changes in the regulation of endothelial barrier function in intact microvessels that have previously been exposed to injury and differences in the regulation of contractile mechanisms between endothelial cells in vivo and in vitro.

KW - Capillary permeability

KW - Endothelial barrier

KW - Endothelial claudins

KW - Endothelial contraction

KW - Endothelial glycocalyx

KW - Pore theory

KW - Rac-1

KW - Small GTP ases

KW - Thrombin

KW - Tight junction

KW - VE-cadherin

UR - http://www.scopus.com/inward/record.url?scp=16244408349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16244408349&partnerID=8YFLogxK

U2 - 10.1080/10739680590894993

DO - 10.1080/10739680590894993

M3 - Article

C2 - 15804971

AN - SCOPUS:16244408349

VL - 12

SP - 17

EP - 31

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 1

ER -