Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation

Mohammad A. Yaseen, Vivek Srinivasan, Sava Sakadžić, Harsha Radhakrishnan, Iwona Gorczynska, Weicheng Wu, James G. Fujimoto, David A. Boas

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Measuring cerebral oxygen delivery and metabolism microscopically is important for interpreting macroscopic functional magnetic resonance imaging (fMRI) data and identifying pathological changes associated with stroke, Alzheimer's disease, and brain injury. Here, we present simultaneous, microscopic measurements of cerebral blood flow (CBF) and oxygen partial pressure (pO2) in cortical microvessels of anesthetized rats under baseline conditions and during somatosensory stimulation. Using a custom-built imaging system, we measured CBF with Fourier-domain optical coherence tomography (OCT), and vascular pO2 with confocal phosphorescence lifetime microscopy. Cerebral blood flow and pO2 measurements displayed heterogeneity over distances irresolvable with fMRI and positron emission tomography. Baseline measurements indicate O2 extraction from pial arterioles and homogeneity of ascending venule pO2 despite large variation in microvessel flows. Oxygen extraction is linearly related to flow in ascending venules, suggesting that flow in ascending venules closely matches oxygen demand of the drained territory. Oxygen partial pressure and relative CBF transients during somatosensory stimulation further indicate arteriolar O 2 extraction and suggest that arterioles contribute to the fMRI blood oxygen level dependent response. Understanding O2 supply on a microscopic level will yield better insight into brain function and the underlying mechanisms of various neuropathologies.

Original languageEnglish (US)
Pages (from-to)1051-1063
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume31
Issue number4
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

Fingerprint

Cerebrovascular Circulation
Cerebral Cortex
Rodentia
Oxygen
Venules
Partial Pressure
Magnetic Resonance Imaging
Arterioles
Microvessels
Optical Coherence Tomography
Positron-Emission Tomography
Brain Injuries
Blood Vessels
Microscopy
Alzheimer Disease
Stroke
Brain

Keywords

  • cerebral blood flow
  • fMRI
  • microscopy
  • optical imaging
  • oxygen

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation. / Yaseen, Mohammad A.; Srinivasan, Vivek; Sakadžić, Sava; Radhakrishnan, Harsha; Gorczynska, Iwona; Wu, Weicheng; Fujimoto, James G.; Boas, David A.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 31, No. 4, 01.04.2011, p. 1051-1063.

Research output: Contribution to journalArticle

Yaseen, Mohammad A. ; Srinivasan, Vivek ; Sakadžić, Sava ; Radhakrishnan, Harsha ; Gorczynska, Iwona ; Wu, Weicheng ; Fujimoto, James G. ; Boas, David A. / Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation. In: Journal of Cerebral Blood Flow and Metabolism. 2011 ; Vol. 31, No. 4. pp. 1051-1063.
@article{b0e673c7a0644950af67d06330b11462,
title = "Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation",
abstract = "Measuring cerebral oxygen delivery and metabolism microscopically is important for interpreting macroscopic functional magnetic resonance imaging (fMRI) data and identifying pathological changes associated with stroke, Alzheimer's disease, and brain injury. Here, we present simultaneous, microscopic measurements of cerebral blood flow (CBF) and oxygen partial pressure (pO2) in cortical microvessels of anesthetized rats under baseline conditions and during somatosensory stimulation. Using a custom-built imaging system, we measured CBF with Fourier-domain optical coherence tomography (OCT), and vascular pO2 with confocal phosphorescence lifetime microscopy. Cerebral blood flow and pO2 measurements displayed heterogeneity over distances irresolvable with fMRI and positron emission tomography. Baseline measurements indicate O2 extraction from pial arterioles and homogeneity of ascending venule pO2 despite large variation in microvessel flows. Oxygen extraction is linearly related to flow in ascending venules, suggesting that flow in ascending venules closely matches oxygen demand of the drained territory. Oxygen partial pressure and relative CBF transients during somatosensory stimulation further indicate arteriolar O 2 extraction and suggest that arterioles contribute to the fMRI blood oxygen level dependent response. Understanding O2 supply on a microscopic level will yield better insight into brain function and the underlying mechanisms of various neuropathologies.",
keywords = "cerebral blood flow, fMRI, microscopy, optical imaging, oxygen",
author = "Yaseen, {Mohammad A.} and Vivek Srinivasan and Sava Sakadžić and Harsha Radhakrishnan and Iwona Gorczynska and Weicheng Wu and Fujimoto, {James G.} and Boas, {David A.}",
year = "2011",
month = "4",
day = "1",
doi = "10.1038/jcbfm.2010.227",
language = "English (US)",
volume = "31",
pages = "1051--1063",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation

AU - Yaseen, Mohammad A.

AU - Srinivasan, Vivek

AU - Sakadžić, Sava

AU - Radhakrishnan, Harsha

AU - Gorczynska, Iwona

AU - Wu, Weicheng

AU - Fujimoto, James G.

AU - Boas, David A.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Measuring cerebral oxygen delivery and metabolism microscopically is important for interpreting macroscopic functional magnetic resonance imaging (fMRI) data and identifying pathological changes associated with stroke, Alzheimer's disease, and brain injury. Here, we present simultaneous, microscopic measurements of cerebral blood flow (CBF) and oxygen partial pressure (pO2) in cortical microvessels of anesthetized rats under baseline conditions and during somatosensory stimulation. Using a custom-built imaging system, we measured CBF with Fourier-domain optical coherence tomography (OCT), and vascular pO2 with confocal phosphorescence lifetime microscopy. Cerebral blood flow and pO2 measurements displayed heterogeneity over distances irresolvable with fMRI and positron emission tomography. Baseline measurements indicate O2 extraction from pial arterioles and homogeneity of ascending venule pO2 despite large variation in microvessel flows. Oxygen extraction is linearly related to flow in ascending venules, suggesting that flow in ascending venules closely matches oxygen demand of the drained territory. Oxygen partial pressure and relative CBF transients during somatosensory stimulation further indicate arteriolar O 2 extraction and suggest that arterioles contribute to the fMRI blood oxygen level dependent response. Understanding O2 supply on a microscopic level will yield better insight into brain function and the underlying mechanisms of various neuropathologies.

AB - Measuring cerebral oxygen delivery and metabolism microscopically is important for interpreting macroscopic functional magnetic resonance imaging (fMRI) data and identifying pathological changes associated with stroke, Alzheimer's disease, and brain injury. Here, we present simultaneous, microscopic measurements of cerebral blood flow (CBF) and oxygen partial pressure (pO2) in cortical microvessels of anesthetized rats under baseline conditions and during somatosensory stimulation. Using a custom-built imaging system, we measured CBF with Fourier-domain optical coherence tomography (OCT), and vascular pO2 with confocal phosphorescence lifetime microscopy. Cerebral blood flow and pO2 measurements displayed heterogeneity over distances irresolvable with fMRI and positron emission tomography. Baseline measurements indicate O2 extraction from pial arterioles and homogeneity of ascending venule pO2 despite large variation in microvessel flows. Oxygen extraction is linearly related to flow in ascending venules, suggesting that flow in ascending venules closely matches oxygen demand of the drained territory. Oxygen partial pressure and relative CBF transients during somatosensory stimulation further indicate arteriolar O 2 extraction and suggest that arterioles contribute to the fMRI blood oxygen level dependent response. Understanding O2 supply on a microscopic level will yield better insight into brain function and the underlying mechanisms of various neuropathologies.

KW - cerebral blood flow

KW - fMRI

KW - microscopy

KW - optical imaging

KW - oxygen

UR - http://www.scopus.com/inward/record.url?scp=79953283190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953283190&partnerID=8YFLogxK

U2 - 10.1038/jcbfm.2010.227

DO - 10.1038/jcbfm.2010.227

M3 - Article

VL - 31

SP - 1051

EP - 1063

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 4

ER -