Microvascular engineering: Dynamic changes in microgel-entrapped vascular cells correlates with higher vasculogenic/angiogenic potential

A. L. Torres, S. J. Bidarra, D. P. Vasconcelos, J. N. Barbosa, E. A. Silva, D. S. Nascimento, C. C. Barrias

Research output: Contribution to journalArticle

Abstract

Successful strategies to promote neovascularization of ischemic tissues are still scarce, being a central priority in regenerative medicine. Microparticles harboring primitive vascular beds are appealing cell delivery candidates for minimally-invasive therapeutic vascularization. However, dynamic cellular alterations associated with in vitro vascular morphogenesis are still elusive. Here, bioengineered microgels guided the assembly of entrapped outgrowth endothelial cells (OEC) and mesenchymal stem cells (MSC) into cohesive vascularized microtissues. During in vitro maturation, OEC formed capillary-like networks enveloped in newly-formed extracellular matrix. Gene expression profiling showed that OEC acquired a mesenchymal-like phenotype, suggesting the occurrence of partial endothelial-to-mesenchymal transition (EndMT), while MSC remained transcriptionally stable. The secretome of entrapped cells became more pro-angiogenic, with no significant alterations of the inflammatory profile. Importantly, matured microgels showed improved cell survival/retention after transplantation in mice, with preservation of capillary-like networks and de novo formation of human vascular structures. These findings support that in vitro priming and morphogenesis of vessel-forming cells improves their vasculogenic/angiogenic potential, which is of therapeutic relevance, shedding some light on the associated mechanisms.

Original languageEnglish (US)
Article number119554
JournalBiomaterials
Volume228
DOIs
StatePublished - Jan 2020

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Endothelial cells
Blood Vessels
Endothelial Cells
Stem cells
Mesenchymal Stromal Cells
Morphogenesis
Regenerative Medicine
Gene Expression Profiling
Gene expression
Extracellular Matrix
Cell Survival
Transplantation
Cells
Tissue
Phenotype
Therapeutics
In Vitro Techniques

Keywords

  • Cell entrapment
  • Cell therapy
  • Injectable biomaterial
  • Morphogenesis
  • Tissue engineering
  • Vascular diseases

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Microvascular engineering : Dynamic changes in microgel-entrapped vascular cells correlates with higher vasculogenic/angiogenic potential. / Torres, A. L.; Bidarra, S. J.; Vasconcelos, D. P.; Barbosa, J. N.; Silva, E. A.; Nascimento, D. S.; Barrias, C. C.

In: Biomaterials, Vol. 228, 119554, 01.2020.

Research output: Contribution to journalArticle

Torres, A. L. ; Bidarra, S. J. ; Vasconcelos, D. P. ; Barbosa, J. N. ; Silva, E. A. ; Nascimento, D. S. ; Barrias, C. C. / Microvascular engineering : Dynamic changes in microgel-entrapped vascular cells correlates with higher vasculogenic/angiogenic potential. In: Biomaterials. 2020 ; Vol. 228.
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