Microvascular abnormalities in sickle cell disease: A computer-assisted intravital microscopy study

Anthony T W Cheung, Peter C Y Chen, Edward C. Larkin, Patricia L. Duong, Sahana Ramanujam, Fern Tablin, Theodore Wun

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The conjunctival microcirculation of 18 homozygous sickle cell disease (SCD) patients during steady-state, painful crisis, and postcrisis conditions was recorded on high-resolution videotapes using intravital microscopy. Selected videotape sequences were subsequently coded, frame-captured, studied, and blindly analyzed using computer-assisted image analysis protocols. At steady-state (baseline), all SCD patients exhibited some of the following morphometric abnormalities: abnormal vessel diameter, comma signs, blood sludging, boxcar blood flow phenomenon, distended vessels, damaged vessels, hemosiderin deposits, vessel tortuosity, and microaneurysms. There was a decrease in vascularity (diminished presence of conjunctival vessels) in SCD patients compared with non-SCD controls, giving the bulbar conjunctiva a "blanched" avascular appearance in most but not all SCD patients during steady-state. Averaged steady-state red cell velocity in SCD patients was slower than in non-SCD controls. During painful crisis, a further decrease in vascularity (caused by flow stoppage in small vessels) and a 36.7% ± 5.2% decrease in large vessel (mostly venular) diameter resulted. In addition, the conjunctival red cell velocities either slowed significantly (6.6% ± 13.1%; P < .01) or were reduced to a trickle (unmeasurable) during crisis. The microvascular changes observed during crisis were transient and reverted to steady-state baseline after resolution of crisis. When combined, intravital microscopy and computer-assisted image analysis (computer-assisted intravital microscopy) represent the availability of a noninvasive tool to quantify microvascular abnormalities in vascular diseases, including sickle cell disease. The ability to identify and relocate the same conjunctival vessels for longitudinal studies uniquely underscores the applicability of this quantitative real-time technology.

Original languageEnglish (US)
Pages (from-to)3999-4005
Number of pages7
JournalBlood
Volume99
Issue number11
DOIs
StatePublished - Jun 1 2002

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Sickle Cell Anemia
Videotape Recording
Computer-Assisted Image Processing
Disease control
Image analysis
Blood
Hemosiderin
Cells
Microcirculation
Conjunctiva
Vascular Diseases
Longitudinal Studies
Intravital Microscopy
Technology
Deposits
Availability

ASJC Scopus subject areas

  • Hematology

Cite this

Microvascular abnormalities in sickle cell disease : A computer-assisted intravital microscopy study. / Cheung, Anthony T W; Chen, Peter C Y; Larkin, Edward C.; Duong, Patricia L.; Ramanujam, Sahana; Tablin, Fern; Wun, Theodore.

In: Blood, Vol. 99, No. 11, 01.06.2002, p. 3999-4005.

Research output: Contribution to journalArticle

Cheung, Anthony T W ; Chen, Peter C Y ; Larkin, Edward C. ; Duong, Patricia L. ; Ramanujam, Sahana ; Tablin, Fern ; Wun, Theodore. / Microvascular abnormalities in sickle cell disease : A computer-assisted intravital microscopy study. In: Blood. 2002 ; Vol. 99, No. 11. pp. 3999-4005.
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abstract = "The conjunctival microcirculation of 18 homozygous sickle cell disease (SCD) patients during steady-state, painful crisis, and postcrisis conditions was recorded on high-resolution videotapes using intravital microscopy. Selected videotape sequences were subsequently coded, frame-captured, studied, and blindly analyzed using computer-assisted image analysis protocols. At steady-state (baseline), all SCD patients exhibited some of the following morphometric abnormalities: abnormal vessel diameter, comma signs, blood sludging, boxcar blood flow phenomenon, distended vessels, damaged vessels, hemosiderin deposits, vessel tortuosity, and microaneurysms. There was a decrease in vascularity (diminished presence of conjunctival vessels) in SCD patients compared with non-SCD controls, giving the bulbar conjunctiva a {"}blanched{"} avascular appearance in most but not all SCD patients during steady-state. Averaged steady-state red cell velocity in SCD patients was slower than in non-SCD controls. During painful crisis, a further decrease in vascularity (caused by flow stoppage in small vessels) and a 36.7{\%} ± 5.2{\%} decrease in large vessel (mostly venular) diameter resulted. In addition, the conjunctival red cell velocities either slowed significantly (6.6{\%} ± 13.1{\%}; P < .01) or were reduced to a trickle (unmeasurable) during crisis. The microvascular changes observed during crisis were transient and reverted to steady-state baseline after resolution of crisis. When combined, intravital microscopy and computer-assisted image analysis (computer-assisted intravital microscopy) represent the availability of a noninvasive tool to quantify microvascular abnormalities in vascular diseases, including sickle cell disease. The ability to identify and relocate the same conjunctival vessels for longitudinal studies uniquely underscores the applicability of this quantitative real-time technology.",
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