Microtubule flux and sliding in mitotic spindles of Drosophila embryos

Ingrid Brust-Mascher, Jonathan M. Scholey

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

We proposed that spindle morphogenesis-in Drosophila embryos involves progression through four transient isometric structures in which a constant spacing of the spindle poles is maintained by a balance of forces generated by multiple microtubule (MT) motors and that tipping this balance drives pole-pole separation. Here we used fluorescent speckle microscopy to evaluate the influence of MT dynamics on the isometric state that persists through metaphase and anaphase A and on pole-pole separation in anaphase B. During metaphase and anaphase A, fluorescent punctae on kinetochore and interpolar MTs flux toward the poles at 0.03 μm/s, too slow to drive chromatid-to-pole motion at 0.11 μm/s, and during anaphase B, fluorescent punctae on interpolar MTs move away from the spindle equator at the same rate as the poles, consistent with MT-MT sliding. Loss of Ncd, a candidate flux motor or brake, did not affect flux in the metaphase/anaphase A isometric state or MT sliding in anaphase B but decreased the duration of the isometric state. Our results suggest that, throughout this isometric state, an outward force exerted on the spindle poles by MT sliding motors is balanced by flux, and that suppression of flux could tip the balance of forces at the onset of anaphase B, allowing MT sliding and polymerization to push the poles apart.

Original languageEnglish (US)
Pages (from-to)3967-3975
Number of pages9
JournalMolecular Biology of the Cell
Volume13
Issue number11
DOIs
StatePublished - Nov 1 2002

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Anaphase
Spindle Apparatus
Microtubules
Drosophila
Embryonic Structures
Metaphase
Spindle Poles
Kinetochores
Chromatids
Morphogenesis
Polymerization
Microscopy

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Microtubule flux and sliding in mitotic spindles of Drosophila embryos. / Brust-Mascher, Ingrid; Scholey, Jonathan M.

In: Molecular Biology of the Cell, Vol. 13, No. 11, 01.11.2002, p. 3967-3975.

Research output: Contribution to journalArticle

Brust-Mascher, Ingrid ; Scholey, Jonathan M. / Microtubule flux and sliding in mitotic spindles of Drosophila embryos. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 11. pp. 3967-3975.
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