Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis

R. F. Hector, B. L. Zimmer, Demosthenes Pappagianis

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37°C with shaking for 96 h, after which the control wells had visible turbidity and endpoints were discernible. Microscopic examination revealed that both control and treatment wells maintained cells predominantly in the spherule-endospore phase of growth.

Original languageEnglish (US)
Pages (from-to)2667-2668
Number of pages2
JournalJournal of Clinical Microbiology
Volume26
Issue number12
StatePublished - 1988

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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