Microspectrofluorometric analysis of surface antigens of murine melanoma and hamster peritoneal cell hybrids: Comparisons of species antigenicity, chromosome number, and tumorigenicity

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Abstract

Somatic cell hybrids from viral fusions of murine melanoma (PAZG) x Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6%) CH chromosome loss but 80% PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and onze PAZG cell, lost 20% of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalOncology
Volume36
Issue number3
StatePublished - 1979

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Hybrid Cells
Surface Antigens
Cricetinae
Melanoma
Chromosomes
Antigens
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 6
Cricetulus
Karyotype
Cell Count

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Microspectrofluorometric analysis of surface antigens of murine melanoma and hamster peritoneal cell hybrids: Comparisons of species antigenicity, chromosome number, and tumorigenicity",
abstract = "Somatic cell hybrids from viral fusions of murine melanoma (PAZG) x Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6{\%}) CH chromosome loss but 80{\%} PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and onze PAZG cell, lost 20{\%} of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells.",
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pages = "101--104",
journal = "Oncology",
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TY - JOUR

T1 - Microspectrofluorometric analysis of surface antigens of murine melanoma and hamster peritoneal cell hybrids

T2 - Comparisons of species antigenicity, chromosome number, and tumorigenicity

AU - Erickson, Kent L

AU - Hu, F.

PY - 1979

Y1 - 1979

N2 - Somatic cell hybrids from viral fusions of murine melanoma (PAZG) x Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6%) CH chromosome loss but 80% PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and onze PAZG cell, lost 20% of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells.

AB - Somatic cell hybrids from viral fusions of murine melanoma (PAZG) x Chinese hamster peritoneal cells (CH) were compared with respect to surface antigenicity, karyotype and tumorigenicity. One line, F57-(9), which arose from the hybridization of two CH cells and one PAZG cell, had slight (6%) CH chromosome loss but 80% PAZG chromosome loss after 10 months in culture. These cells expressed CH antigens strongly and PAZG antigens weakly. In comparison, another hybrid, F57-(7), formed from one CH and onze PAZG cell, lost 20% of its chromosomes after 10 months in vitro. These cells had a stronger expression of PAZG antigens and weaker expression of CH antigens than F57-(9). These findings indicate a direct relationship between chromosome number and antigenicity; tumorigenicity, however, does not appear to depend on the chromosome numbers of the parental cells.

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