Microsomal Aryl Hydrocarbon Hydroxylase Comparison of the Direct, Indirect and Radiometric Assays

M. S. Denison, M. Murray, C. F. Wilkinson

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The direct fluorometric assay of aryl hydrocarbon hydroxylase has been compared to the more commonly used indirect fluorometric and radiometric assays. Although rat hepatic microsomal activities measured by the direct assay were consistently higher than those obtained by the other assays, the relative changes in activity following enzyme induction and/or inhibition were similar. The direct assay provides an accurate and rapid measure of aryl hydrocarbon hydroxylase activity and avoids several problems inherent in the indirect and radiometric assays.

Original languageEnglish (US)
Pages (from-to)381-391
Number of pages11
JournalAnalytical Letters
Volume16
Issue number5
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Keywords

  • AHH
  • Aryl hydrocarbon hydroxylase
  • cytochrome P-450

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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