Microscopy with ultraviolet surface excitation for rapid slide-free histology

Farzad Fereidouni, Zachary T. Harmany, Miao Tian, Austin Todd, John A. Kintner, John Douglas Mcpherson, Alexander D Borowsky, John W Bishop, Mirna Lechpammer, Stavros G. Demos, Richard M Levenson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Histological examination of tissues is central to the diagnosis and management of neoplasms and many other diseases and is a foundational technique for preclinical and basic research. However, commonly used bright-field microscopy requires prior preparation of micrometre-thick tissue sections mounted on glass slides - a process that can require hours or days, contributes to cost and delays access to critical information. Here, we introduce a simple, non-destructive slide-free technique that, within minutes, provides high-resolution diagnostic histological images resembling those obtained from conventional haematoxylin and eosin histology. The approach, which we named microscopy with ultraviolet surface excitation (MUSE), can also generate shape and colour-contrast information. MUSE relies on ~280 nm ultraviolet light to restrict the excitation of conventional fluorescent stains to tissue surfaces and it has no significant effects on downstream molecular assays (including fluorescence in situ hybridization and RNA sequencing). MUSE promises to improve the speed and efficiency of patient care in both state-of-the-art and low-resource settings and to provide opportunities for rapid histology in research.

Original languageEnglish (US)
Pages (from-to)957-966
Number of pages10
JournalNature Biomedical Engineering
Volume1
Issue number12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Ultraviolet Microscopy
Histology
Microscopic examination
Tissue
RNA Sequence Analysis
Access to Information
Hematoxylin
Ultraviolet Rays
Eosine Yellowish-(YS)
Fluorescence In Situ Hybridization
Research
Glass
Microscopy
Patient Care
Coloring Agents
Color
RNA
Costs and Cost Analysis
Assays
Fluorescence

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Computer Science Applications

Cite this

Microscopy with ultraviolet surface excitation for rapid slide-free histology. / Fereidouni, Farzad; Harmany, Zachary T.; Tian, Miao; Todd, Austin; Kintner, John A.; Mcpherson, John Douglas; Borowsky, Alexander D; Bishop, John W; Lechpammer, Mirna; Demos, Stavros G.; Levenson, Richard M.

In: Nature Biomedical Engineering, Vol. 1, No. 12, 01.12.2017, p. 957-966.

Research output: Contribution to journalArticle

Fereidouni, Farzad ; Harmany, Zachary T. ; Tian, Miao ; Todd, Austin ; Kintner, John A. ; Mcpherson, John Douglas ; Borowsky, Alexander D ; Bishop, John W ; Lechpammer, Mirna ; Demos, Stavros G. ; Levenson, Richard M. / Microscopy with ultraviolet surface excitation for rapid slide-free histology. In: Nature Biomedical Engineering. 2017 ; Vol. 1, No. 12. pp. 957-966.
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