Abstract
Nitric oxide (NO) appears in the exhaled breath and is elevated in inflammatory diseases. We developed a steady-state mathematical model of the bronchial mucosa for normal small and large airways to understand NO and S-nitrosoglutathione (GSNO) kinetics and transport using data from the existing literature. Our model predicts that mean steady-state NO and GSNO concentrations for large airways (generation 1) are 2.68 nM and 113 pM, respectively, in the epithelial cells and 0.11 nM (∼66 ppb) and 507 nM in the mucus. For small airways (generation 15), the mean concentrations of NO and GSNO, respectively, are 0.26 nM and 21 pM in the epithelial cells and 0.02 nM (∼12 ppb) and 132 nM in the mucus. The concentrations in the mucus compare favorably to experimentally measured values. We conclude that 1) the majority of free NO in the mucus, and thus exhaled NO, is due to diffusion of free NO from the epithelial cell and 2) the heterogeneous airway contribution to exhaled NO is due to heterogeneous airway geometries, such as epithelium and mucus thickness.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 777-788 |
| Number of pages | 12 |
| Journal | Journal of Applied Physiology |
| Volume | 90 |
| Issue number | 3 |
| State | Published - Mar 6 2001 |
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Keywords
- Exhalation
- Inflammation
- Nitric oxide
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Microscopic modeling of NO and S-nitrosoglutathione kinetics and transport in human airways. / Shin, Hye Won; George, Steven.
In: Journal of Applied Physiology, Vol. 90, No. 3, 06.03.2001, p. 777-788.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microscopic modeling of NO and S-nitrosoglutathione kinetics and transport in human airways
AU - Shin, Hye Won
AU - George, Steven
PY - 2001/3/6
Y1 - 2001/3/6
N2 - Nitric oxide (NO) appears in the exhaled breath and is elevated in inflammatory diseases. We developed a steady-state mathematical model of the bronchial mucosa for normal small and large airways to understand NO and S-nitrosoglutathione (GSNO) kinetics and transport using data from the existing literature. Our model predicts that mean steady-state NO and GSNO concentrations for large airways (generation 1) are 2.68 nM and 113 pM, respectively, in the epithelial cells and 0.11 nM (∼66 ppb) and 507 nM in the mucus. For small airways (generation 15), the mean concentrations of NO and GSNO, respectively, are 0.26 nM and 21 pM in the epithelial cells and 0.02 nM (∼12 ppb) and 132 nM in the mucus. The concentrations in the mucus compare favorably to experimentally measured values. We conclude that 1) the majority of free NO in the mucus, and thus exhaled NO, is due to diffusion of free NO from the epithelial cell and 2) the heterogeneous airway contribution to exhaled NO is due to heterogeneous airway geometries, such as epithelium and mucus thickness.
AB - Nitric oxide (NO) appears in the exhaled breath and is elevated in inflammatory diseases. We developed a steady-state mathematical model of the bronchial mucosa for normal small and large airways to understand NO and S-nitrosoglutathione (GSNO) kinetics and transport using data from the existing literature. Our model predicts that mean steady-state NO and GSNO concentrations for large airways (generation 1) are 2.68 nM and 113 pM, respectively, in the epithelial cells and 0.11 nM (∼66 ppb) and 507 nM in the mucus. For small airways (generation 15), the mean concentrations of NO and GSNO, respectively, are 0.26 nM and 21 pM in the epithelial cells and 0.02 nM (∼12 ppb) and 132 nM in the mucus. The concentrations in the mucus compare favorably to experimentally measured values. We conclude that 1) the majority of free NO in the mucus, and thus exhaled NO, is due to diffusion of free NO from the epithelial cell and 2) the heterogeneous airway contribution to exhaled NO is due to heterogeneous airway geometries, such as epithelium and mucus thickness.
KW - Exhalation
KW - Inflammation
KW - Nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=0035101798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035101798&partnerID=8YFLogxK
M3 - Article
C2 - 11181583
AN - SCOPUS:0035101798
VL - 90
SP - 777
EP - 788
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 3
ER -