Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua)

Michelle P. Cubilla, Leonilda C. Santos, Wanderlei de Moraes, Zalmir S. Cubas, Christian M. Leutenegger, Marko Estrada, Le Ann L. Lindsay, Edvaldo S. Trindade, Célia Regina C. Franco, Rafael F.C. Vieira, Alexander W. Biondo, Jane E Sykes

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hemoplasmas were detected in two apparently healthy captive South American coatis (Nasua nasua) from southern Brazil during an investigation for vector-borne pathogens. Blood was subjected to packed cell volume (PCV) determination, a commercial real-time PCR panel for the detection of Anaplasma spp., Babesia spp., Bartonella spp., Hepatozoon spp., Leishmania spp., Mycoplasma haemofelis, ‘Candidatus Mycoplasma turicensis’, ‘Candidatus Mycoplasma haemominutum’, Neorickettsia risticii, Rickettsia rickettsii and Leptospira spp., and a pan-hemoplasma conventional PCR assay. PCV was normal, but both coatis tested positive for hemoplasmas and negative for all the remaining pathogens tested. Using different techniques for microscopy (light, confocal or SEM), structures compatible with hemoplasmas were identified. Sequencing of the 16S rRNA gene identified an organism resembling Mycoplasma haemofelis and another hemotropic Mycoplasma sp., with a sequence identity of 96.8% to a Mycoplasma sp. previously detected in capybaras.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume53
DOIs
StatePublished - Aug 1 2017

Fingerprint

Procyonidae
Mycoplasma
Mycoplasma haemofelis
hematocrit
Mycoplasma haemominutum
Neorickettsia risticii
Rickettsia rickettsii
Hydrochaeris hydrochaeris
Hepatozoon
Bartonella
Anaplasma
Babesia
Leptospira
pathogens
Leishmania
Cell Size
light microscopy
quantitative polymerase chain reaction
ribosomal RNA
Brazil

Keywords

  • Hemoplasma
  • Mycoplasma haemofelis
  • Vector-borne disease
  • Wild mammal

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua). / Cubilla, Michelle P.; Santos, Leonilda C.; de Moraes, Wanderlei; Cubas, Zalmir S.; Leutenegger, Christian M.; Estrada, Marko; Lindsay, Le Ann L.; Trindade, Edvaldo S.; Franco, Célia Regina C.; Vieira, Rafael F.C.; Biondo, Alexander W.; Sykes, Jane E.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 53, 01.08.2017, p. 19-25.

Research output: Contribution to journalArticle

Cubilla, MP, Santos, LC, de Moraes, W, Cubas, ZS, Leutenegger, CM, Estrada, M, Lindsay, LAL, Trindade, ES, Franco, CRC, Vieira, RFC, Biondo, AW & Sykes, JE 2017, 'Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua)', Comparative Immunology, Microbiology and Infectious Diseases, vol. 53, pp. 19-25. https://doi.org/10.1016/j.cimid.2017.06.004
Cubilla, Michelle P. ; Santos, Leonilda C. ; de Moraes, Wanderlei ; Cubas, Zalmir S. ; Leutenegger, Christian M. ; Estrada, Marko ; Lindsay, Le Ann L. ; Trindade, Edvaldo S. ; Franco, Célia Regina C. ; Vieira, Rafael F.C. ; Biondo, Alexander W. ; Sykes, Jane E. / Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua). In: Comparative Immunology, Microbiology and Infectious Diseases. 2017 ; Vol. 53. pp. 19-25.
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