Microscope slide electrode chamber for nanosecond, megavolt-per-meter biological investigations

Y. Sun, P. T. Vernier, M. Behrend, Laura Marcu, M. A. Gundersen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Nanosecond pulsed electric fields pass through the external membranes of biological cells (which have typical response times much greater than nanoseconds) and perturb fast-responding intracellular structures and processes. To enable real-time imaging and investigation of these phenomena, a microchamber with integral electrode walls and optical path for observing individual cells exposed to electric pulses was designed and fabricated utilizing photolithographic and microelectronic fabrication methods. SU-8 photoresist was patterned to form straight sidewalls 10 to 30 μm in height, and gold was deposited on the opposing walls and the top for a conductive, non-reactive electrode surface. Results from observations with the microchamber in real-time electroperturbation imaging experiments include intracellular calcium bursts and membrane phospholipid translocation. Real-time nanoelectropulse investigations with microfabricated imaging tools open new pathways for the study and engineering of biological systems.

Original languageEnglish (US)
Title of host publication2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
EditorsM. Laudon, B. Romanowicz
Pages485-488
Number of pages4
Volume1
StatePublished - 2004
Event2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 - Boston, MA, United States
Duration: Mar 7 2004Mar 11 2004

Other

Other2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
CountryUnited States
CityBoston, MA
Period3/7/043/11/04

Keywords

  • Electroperturbation
  • Liftoff
  • Megavolt electric field
  • Microchamber
  • Microelectronic fabrication technology
  • Microscopy
  • SU-8 photoresist

ASJC Scopus subject areas

  • Engineering(all)

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