Abstract
The goal of this study was to develop an innovative approach to image gene expression in intact 3D tissues. Imaging gene expression of individual cells in 3D tissues is expected to have a significant impact on both clinical diagnostic applications and fundamental biological science and engineering applications in a laboratory setting. To achieve this goal, we have developed an integrated approach that combines: 1) microneedle-based minimally invasive intra-tissue delivery of oligonucleotide probes and Streptolysin O (SLO) or CPP; 2) SLO as a pore forming permeation enhancer to enable intracellular delivery of oligonucleotide probes and CPP peptides can also transport conjugated cargo in cells; and 3) fluorescence resonance energy transfer (FRET) pair of ON probes to improve specificity and sensitivity of RNA detection in tissue models. The results of this study demonstrate uniform coating and rapid release of ON probes from microneedles in a tissue environment. Microneedle assisted delivery of ON probes in 3D tissue does not result in cell damage and the ON probes are uniformly delivered in the tissue. The results also demonstrate the feasibility of FRET imaging of ON probes in 3D tissue and highlight the potential for imaging 28-s rRNA in individual living cells.
Original language | English (US) |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 8725 |
DOIs | |
State | Published - 2013 |
Externally published | Yes |
Event | 2013 Micro- and Nanotechnology Sensors, Systems, and Applications V Conference - Baltimore, MD, United States Duration: Apr 29 2013 → May 3 2013 |
Other
Other | 2013 Micro- and Nanotechnology Sensors, Systems, and Applications V Conference |
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Country/Territory | United States |
City | Baltimore, MD |
Period | 4/29/13 → 5/3/13 |
Keywords
- FRET imaging
- Gene expression
- Microneedle
- Oligonucleotide delivery
- Streptolysin O
- Tissue
ASJC Scopus subject areas
- Applied Mathematics
- Computer Science Applications
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics