Microsatellite typing of the rhesus macaque MHC region

Cecilia Penedo, Ronald E. Bontrop, Corrine M.C. Heijmans, Nel Otting, Riet Noort, Annemiek J.M. Rouweler, Nanine De Groot, Natasja G. De Groot, Thea Ward, Gaby G.M. Doxiadis

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu-DRB loci, the Mamu-A and -B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu-DR and -DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.

Original languageEnglish (US)
Pages (from-to)198-209
Number of pages12
JournalImmunogenetics
Volume57
Issue number3-4
DOIs
StatePublished - May 1 2005

Fingerprint

Major Histocompatibility Complex
Macaca mulatta
Microsatellite Repeats
Haplotypes
Dichlororibofuranosylbenzimidazole
MHC Class I Genes
Molecular Typing
Serotyping
MHC Class II Genes
Histocompatibility Antigens
Linkage Disequilibrium
Pedigree
Gene Frequency
Breeding
Biomedical Research
Population
Genes

Keywords

  • Evolution
  • Haplotype
  • MHC
  • Microsatellites
  • Non-human primates

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Penedo, C., Bontrop, R. E., Heijmans, C. M. C., Otting, N., Noort, R., Rouweler, A. J. M., ... Doxiadis, G. G. M. (2005). Microsatellite typing of the rhesus macaque MHC region. Immunogenetics, 57(3-4), 198-209. https://doi.org/10.1007/s00251-005-0787-1

Microsatellite typing of the rhesus macaque MHC region. / Penedo, Cecilia; Bontrop, Ronald E.; Heijmans, Corrine M.C.; Otting, Nel; Noort, Riet; Rouweler, Annemiek J.M.; De Groot, Nanine; De Groot, Natasja G.; Ward, Thea; Doxiadis, Gaby G.M.

In: Immunogenetics, Vol. 57, No. 3-4, 01.05.2005, p. 198-209.

Research output: Contribution to journalArticle

Penedo, C, Bontrop, RE, Heijmans, CMC, Otting, N, Noort, R, Rouweler, AJM, De Groot, N, De Groot, NG, Ward, T & Doxiadis, GGM 2005, 'Microsatellite typing of the rhesus macaque MHC region', Immunogenetics, vol. 57, no. 3-4, pp. 198-209. https://doi.org/10.1007/s00251-005-0787-1
Penedo C, Bontrop RE, Heijmans CMC, Otting N, Noort R, Rouweler AJM et al. Microsatellite typing of the rhesus macaque MHC region. Immunogenetics. 2005 May 1;57(3-4):198-209. https://doi.org/10.1007/s00251-005-0787-1
Penedo, Cecilia ; Bontrop, Ronald E. ; Heijmans, Corrine M.C. ; Otting, Nel ; Noort, Riet ; Rouweler, Annemiek J.M. ; De Groot, Nanine ; De Groot, Natasja G. ; Ward, Thea ; Doxiadis, Gaby G.M. / Microsatellite typing of the rhesus macaque MHC region. In: Immunogenetics. 2005 ; Vol. 57, No. 3-4. pp. 198-209.
@article{126bbe9864dd41b1b3f6c8275f9b9bd9,
title = "Microsatellite typing of the rhesus macaque MHC region",
abstract = "To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu-DRB loci, the Mamu-A and -B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu-DR and -DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.",
keywords = "Evolution, Haplotype, MHC, Microsatellites, Non-human primates",
author = "Cecilia Penedo and Bontrop, {Ronald E.} and Heijmans, {Corrine M.C.} and Nel Otting and Riet Noort and Rouweler, {Annemiek J.M.} and {De Groot}, Nanine and {De Groot}, {Natasja G.} and Thea Ward and Doxiadis, {Gaby G.M.}",
year = "2005",
month = "5",
day = "1",
doi = "10.1007/s00251-005-0787-1",
language = "English (US)",
volume = "57",
pages = "198--209",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Microsatellite typing of the rhesus macaque MHC region

AU - Penedo, Cecilia

AU - Bontrop, Ronald E.

AU - Heijmans, Corrine M.C.

AU - Otting, Nel

AU - Noort, Riet

AU - Rouweler, Annemiek J.M.

AU - De Groot, Nanine

AU - De Groot, Natasja G.

AU - Ward, Thea

AU - Doxiadis, Gaby G.M.

PY - 2005/5/1

Y1 - 2005/5/1

N2 - To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu-DRB loci, the Mamu-A and -B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu-DR and -DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.

AB - To improve the results gained by serotyping rhesus macaque major histocompatibility complex (MHC) antigens, molecular typing techniques have been established for class I and II genes. Like the rhesus macaque Mamu-DRB loci, the Mamu-A and -B are not only polymorphic but also polygenic. As a consequence, sequence-based typing of these genes is time-consuming. Therefore, eight MHC-linked microsatellites, or short tandem repeats (STRs), were evaluated for their use in haplotype characterization. Polymorphism analyses in rhesus macaques of Indian and Chinese origin showed high STR allelic diversity in both populations but different patterns of allele frequency distribution between the groups. Pedigree data for class I and II loci and the eight STRs allowed us to determine extended MHC haplotypes in rhesus macaque breeding groups. STR sequencing and comparisons with the complete rhesus macaque MHC genomic map allowed the exact positioning of the markers. Strong linkage disequilibria were observed between Mamu-DR and -DQ loci and adjacent STRs. Microsatellite typing provides an efficient, robust, and quick method of genotyping and deriving MHC haplotypes for rhesus macaques regardless of their geographical origin. The incorporation of MHC-linked STRs into routine genetic tests will contribute to efforts to improve the genetic characterization of the rhesus macaque for biomedical research and can provide comparative information about the evolution of the MHC region.

KW - Evolution

KW - Haplotype

KW - MHC

KW - Microsatellites

KW - Non-human primates

UR - http://www.scopus.com/inward/record.url?scp=20144378498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144378498&partnerID=8YFLogxK

U2 - 10.1007/s00251-005-0787-1

DO - 10.1007/s00251-005-0787-1

M3 - Article

C2 - 15900491

AN - SCOPUS:20144378498

VL - 57

SP - 198

EP - 209

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 3-4

ER -