Microsatellite markers associated with quantitative trait loci controlling antibody response to Escherichia coli and Salmonella enteritidis in young broilers

R. Yunis, E. D. Heller, J. Hillel, A. Cahaner

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A unique resource population was produced to facilitate detection of microsatellite markers associated with quantitative trait loci controlling antibody (Ab) response in broiler chickens. Three F1 males were produced by mating two lines divergently selected on Ab response to Escherichia coli vaccination. Each F1 male was mated with females from four genetic backgrounds: F1, high-Ab line (HH), low-Ab line and commercial line, producing three resource families, each with four progeny types. About 1700 chicks were immunized with E. coli and Salmonella enteritidis vaccines. Selective genotyping was conducted on the individuals with highest or lowest average Ab to E. coli and S. enteritidis within each progeny type in each sire family. Twelve markers were significantly associated with Ab to E. coli and six of them were also associated with Ab to S. enteritidis, mostly exhibiting a similar low effect (∼ 0.35 phenotypic SD) in all progeny types. Four markers exhibited a highly significant and much larger effect (∼ 1.7 SD), but only in progeny of females from the HH, suggesting that a backcross to the high parental line should be preferred over the commonly used F2 population. Results from two markers suggested a quantitative trait locus on chromosome 2 around 400 cM. The marker MCW0083, significant in two sire families, is closely linked to the bone morphogenetic protein 2 (BMP2) gene, known to be associated with the control of T-cell transformation in humans.

Original languageEnglish (US)
Pages (from-to)407-414
Number of pages8
JournalAnimal Genetics
Volume33
Issue number6
DOIs
StatePublished - Dec 2002
Externally publishedYes

Fingerprint

Salmonella enteritidis
Quantitative Trait Loci
Salmonella Enteritidis
Microsatellite Repeats
Antibody Formation
quantitative trait loci
broiler chickens
microsatellite repeats
Escherichia coli
antibodies
Antibodies
Salmonella Vaccines
sires
Bone Morphogenetic Protein 2
Chromosomes, Human, Pair 2
Population
bone morphogenetic proteins
Chickens
Vaccination
T-Lymphocytes

Keywords

  • Antibody
  • Broiler
  • Genetic background
  • Immune response
  • Microsatellite markers
  • QTL

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

Microsatellite markers associated with quantitative trait loci controlling antibody response to Escherichia coli and Salmonella enteritidis in young broilers. / Yunis, R.; Heller, E. D.; Hillel, J.; Cahaner, A.

In: Animal Genetics, Vol. 33, No. 6, 12.2002, p. 407-414.

Research output: Contribution to journalArticle

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