Abstract
Skin fibrosis is a chronic debilitating feature of several skin diseases that lead to characteristic increases in dermal fibroblast proliferation and collagen deposition through upregulation in components of the transforming growth factor beta (TGF-B)/SMAD pathway. In contrast to ultraviolet phototherapy, high-fluence light-emitting diode-generated red light (HF-LED-RL, 633 ± 15 nm) is a safe, economic and non-invasive therapy with in vitro evidence that supports modulation of the key cellular characteristics involved in the pathogenesis of skin fibrosis. Limited data exists pertaining to the effects of HF-LED-RL on human skin fibroblast microRNA (miRNA). Herein, we explored the effects of HF-LED-RL on fibroblast miRNA levels using RNA-seq and miRNA expression analysis. Using RNA-seq analysis we found that HF-LED-RL at 320 and 640 J/cm2 increased transcription of key miRNA that are involved in skin fibrosis including miRNA-29, miRNA-196a and Let-7a, and decreased transcription of miRNA-21, miRNA-23b and miRNA-31. These microRNA findings provide insight into the molecular underpinnings of HF-LED-RL and highlight potential therapeutic targets of interest for the treatment of skin fibrosis. Additional research on the specific molecular mechanisms underlying HF-LED-RL effects on fibroblasts may provide further mechanistic insight into this therapy and may reveal additional future therapeutic targets for skin fibrosis.
| Original language | English (US) |
|---|---|
| Article number | e201800207 |
| Journal | Journal of Biophotonics |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- fibroblasts
- LED, light emitting diode
- phototherapy
- red light
- RNA-seq
- Skin fibrosis
- transcriptome
ASJC Scopus subject areas
- Chemistry(all)
- Materials Science(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Engineering(all)
- Physics and Astronomy(all)
Cite this
MicroRNA expression analysis of human skin fibroblasts treated with high-fluence light-emitting diode-red light. / Mamalis, Andrew; Koo, Eugene; Tepper, Clifford G; Jagdeo, Jared.
In: Journal of Biophotonics, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNA expression analysis of human skin fibroblasts treated with high-fluence light-emitting diode-red light
AU - Mamalis, Andrew
AU - Koo, Eugene
AU - Tepper, Clifford G
AU - Jagdeo, Jared
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Skin fibrosis is a chronic debilitating feature of several skin diseases that lead to characteristic increases in dermal fibroblast proliferation and collagen deposition through upregulation in components of the transforming growth factor beta (TGF-B)/SMAD pathway. In contrast to ultraviolet phototherapy, high-fluence light-emitting diode-generated red light (HF-LED-RL, 633 ± 15 nm) is a safe, economic and non-invasive therapy with in vitro evidence that supports modulation of the key cellular characteristics involved in the pathogenesis of skin fibrosis. Limited data exists pertaining to the effects of HF-LED-RL on human skin fibroblast microRNA (miRNA). Herein, we explored the effects of HF-LED-RL on fibroblast miRNA levels using RNA-seq and miRNA expression analysis. Using RNA-seq analysis we found that HF-LED-RL at 320 and 640 J/cm2 increased transcription of key miRNA that are involved in skin fibrosis including miRNA-29, miRNA-196a and Let-7a, and decreased transcription of miRNA-21, miRNA-23b and miRNA-31. These microRNA findings provide insight into the molecular underpinnings of HF-LED-RL and highlight potential therapeutic targets of interest for the treatment of skin fibrosis. Additional research on the specific molecular mechanisms underlying HF-LED-RL effects on fibroblasts may provide further mechanistic insight into this therapy and may reveal additional future therapeutic targets for skin fibrosis.
AB - Skin fibrosis is a chronic debilitating feature of several skin diseases that lead to characteristic increases in dermal fibroblast proliferation and collagen deposition through upregulation in components of the transforming growth factor beta (TGF-B)/SMAD pathway. In contrast to ultraviolet phototherapy, high-fluence light-emitting diode-generated red light (HF-LED-RL, 633 ± 15 nm) is a safe, economic and non-invasive therapy with in vitro evidence that supports modulation of the key cellular characteristics involved in the pathogenesis of skin fibrosis. Limited data exists pertaining to the effects of HF-LED-RL on human skin fibroblast microRNA (miRNA). Herein, we explored the effects of HF-LED-RL on fibroblast miRNA levels using RNA-seq and miRNA expression analysis. Using RNA-seq analysis we found that HF-LED-RL at 320 and 640 J/cm2 increased transcription of key miRNA that are involved in skin fibrosis including miRNA-29, miRNA-196a and Let-7a, and decreased transcription of miRNA-21, miRNA-23b and miRNA-31. These microRNA findings provide insight into the molecular underpinnings of HF-LED-RL and highlight potential therapeutic targets of interest for the treatment of skin fibrosis. Additional research on the specific molecular mechanisms underlying HF-LED-RL effects on fibroblasts may provide further mechanistic insight into this therapy and may reveal additional future therapeutic targets for skin fibrosis.
KW - fibroblasts
KW - LED, light emitting diode
KW - phototherapy
KW - red light
KW - RNA-seq
KW - Skin fibrosis
KW - transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85060928105&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060928105&partnerID=8YFLogxK
U2 - 10.1002/jbio.201800207
DO - 10.1002/jbio.201800207
M3 - Article
C2 - 30182520
AN - SCOPUS:85060928105
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
M1 - e201800207
ER -