microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma

Ke Cao, Jingjing Li, Jia Chen, Li Qian, Aijun Wang, Xiang Chen, Wei Xiong, Jintian Tang, Shijie Tang, Yong Chen, Yao Chen, Yan Cheng, Jianda Zhou

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1α), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR- 33a-5p in WM451 cells, accompanied by a decreased level of glycolysis. In contrast, cell proliferation was upregulated after inhibition of miR-33a-5p in WM35 cells, accompanied by increased glycolysis. Overexpression of miR-33a-5p enhanced the sensitivity of melanoma cells to X-radiation by MTT assay, while downregulation of miR-33a-5p had the opposite effects. Finally, in vivo experiments with xenografts in nude mice confirmed that high expression of miR-33a-5p in tumor cells increased radiosensitivity via inhibiting glycolysis. In conclusions, miR-33a-5p promotes radiosensitivity by negatively regulating glycolysis in melanoma.

Original languageEnglish (US)
Pages (from-to)83660-83672
Number of pages13
JournalOncotarget
Volume8
Issue number48
DOIs
StatePublished - 2017

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Radiation Tolerance
Glycolysis
MicroRNAs
Melanoma
Down-Regulation
Cell Proliferation
Heterografts
Nude Mice
Neoplasms
X-Rays
Genes

Keywords

  • Glucose
  • HIF-1α
  • Lactate dehydrogenase A
  • MicroRNA
  • Radiation

ASJC Scopus subject areas

  • Oncology

Cite this

microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma. / Cao, Ke; Li, Jingjing; Chen, Jia; Qian, Li; Wang, Aijun; Chen, Xiang; Xiong, Wei; Tang, Jintian; Tang, Shijie; Chen, Yong; Chen, Yao; Cheng, Yan; Zhou, Jianda.

In: Oncotarget, Vol. 8, No. 48, 2017, p. 83660-83672.

Research output: Contribution to journalArticle

Cao, K, Li, J, Chen, J, Qian, L, Wang, A, Chen, X, Xiong, W, Tang, J, Tang, S, Chen, Y, Chen, Y, Cheng, Y & Zhou, J 2017, 'microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma', Oncotarget, vol. 8, no. 48, pp. 83660-83672. https://doi.org/10.18632/oncotarget.19014
Cao, Ke ; Li, Jingjing ; Chen, Jia ; Qian, Li ; Wang, Aijun ; Chen, Xiang ; Xiong, Wei ; Tang, Jintian ; Tang, Shijie ; Chen, Yong ; Chen, Yao ; Cheng, Yan ; Zhou, Jianda. / microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma. In: Oncotarget. 2017 ; Vol. 8, No. 48. pp. 83660-83672.
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abstract = "Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1α), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR- 33a-5p in WM451 cells, accompanied by a decreased level of glycolysis. In contrast, cell proliferation was upregulated after inhibition of miR-33a-5p in WM35 cells, accompanied by increased glycolysis. Overexpression of miR-33a-5p enhanced the sensitivity of melanoma cells to X-radiation by MTT assay, while downregulation of miR-33a-5p had the opposite effects. Finally, in vivo experiments with xenografts in nude mice confirmed that high expression of miR-33a-5p in tumor cells increased radiosensitivity via inhibiting glycolysis. In conclusions, miR-33a-5p promotes radiosensitivity by negatively regulating glycolysis in melanoma.",
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