Micropulser for real-time microscopy of cell electroperturbation

Matthew Behrend, Andras Kuthi, P. Thomas Vernier, Laura Marcu, Cheryl Craft, Martin Gundersen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Scopus citations

Abstract

A miniature solid state pulse generator (≈400 V) is designed for the electroperturbation of biological cells in solution. Design emphasis is placed on compactness and the control over pulse shape with the goal of targeting specific cell responses, including apoptosis. Cell electroperturbation with nanosecond pulses is typically performed on a batch of cells in a cuvette with volume of less than 1 mL. The desire to observe in real time morphological changes and electrical potential gradients necessitates a design appropriate for optical and electrical studies. We describe here a "micropulser" designed to deliver pulses of several hundred volts to a narrow channel of cells on a microscope slide. The pulse generator, based on a single fast power MOSFET, forms square pulses of variable width from 15 ns to DC. The pulse generator unit and slide holder are compact and designed for optical access and monitoring.

Original languageEnglish (US)
Title of host publicationIEEE Conference Record of Power Modulator Symposium
EditorsL. Gordon
Pages358-361
Number of pages4
StatePublished - 2002
Externally publishedYes
EventConference Record of the Twenty-Fifth International Power Modulator Symposium and 2002 High-Voltage Workshop - Hollywood, CA, United States
Duration: Jun 30 2002Jul 3 2002

Other

OtherConference Record of the Twenty-Fifth International Power Modulator Symposium and 2002 High-Voltage Workshop
CountryUnited States
CityHollywood, CA
Period6/30/027/3/02

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Behrend, M., Kuthi, A., Vernier, P. T., Marcu, L., Craft, C., & Gundersen, M. (2002). Micropulser for real-time microscopy of cell electroperturbation. In L. Gordon (Ed.), IEEE Conference Record of Power Modulator Symposium (pp. 358-361)