Micropatterning of bioactive heparin-based hydrogels

Sunny Satish Shah, Mihye Kim, Katelyn Cahill-Thompson, Giyoong Tae, Alexander Revzin

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

This paper describes a UV photopatterning of bioactive heparin-based hydrogels on glass substrates. In this approach, hydrogel micropatterns were formed by UV-initiated thiol-ene reaction between thiolated heparin and diacrylated poly(ethylene) glycol (PEG-DA). Analysis of gelation kinetics showed that photo-crosslinked hydrogels formed faster and were stronger when compared to hydrogels formed by competing Michael addition reaction. To highlight bioactivity of heparin-PEG hybrid gels, hepatocyte growth factor (HGF) was mixed into prepolymer solution prior to hydrogel patterning. Immunostaining showed that HGF was retained after 5 days in the hybrid heparin-PEG hydrogel microstructures but was rapidly released from pure PEG gel microstructures. In a set of experiments further highlighting bioactivity of microfabricated heparin-based hydrogel, primary rat hepatocytes were cultured next to heparin and pure PEG hydrogel disks (̃500 mm in diameter). ELISA analysis revealed that hepatocytes residing next to heparin-based hydrogels were producing ̃4 times more albumin at day 7 compared to cells cultured next to inert PEG hydrogels. In the future, microfabricated heparin-based hydrogels described in this paper will be employed for designing cellular microenvironment in vitro and as vehicles for cell transplantation in vivo.

Original languageEnglish (US)
Pages (from-to)3133-3140
Number of pages8
JournalSoft Matter
Volume7
Issue number7
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

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    Shah, S. S., Kim, M., Cahill-Thompson, K., Tae, G., & Revzin, A. (2011). Micropatterning of bioactive heparin-based hydrogels. Soft Matter, 7(7), 3133-3140. https://doi.org/10.1039/c0sm00771d