Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system

Gulnaz Stybayeva, He Zhu, Erlan Ramanculov, Satya Dandekar, Michael George, Alexander Revzin

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)-infected T-cells. Our studies seek to recapitulate, in vitro, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function. Here, we describe a novel approach for creating co-cultures of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells where both cell types are positioned on the same surface in a price spatial configuration (micropattern). This co-culture method simplified observation/monitoring of the two cell types and was particularly suited for laser microdissection-based retrieval of the desired cells for downstream gene expressions studies. DNA microarray analysis of epithelial cells retrieved from co-cultures with HIV-1-infected vs. uninfected Jurkat cells revealed that epithelial cells from HIV-infected co-cultures exhibited gene expression patterns consistent with disruption of epithelial barrier formation. Overall, the micropatterned co-culture system described here is envisioned as a valuable new tool for delineating how HIV and other infections contribute to dysfunction of mucosal epithelium.

Original languageEnglish (US)
Pages (from-to)575-580
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number3
DOIs
StatePublished - Mar 13 2009

Keywords

  • Antibody microarrays
  • Epithelial cells
  • HIV enteropathy
  • Micropatterned co-cultures
  • Mucosal immune system
  • T-lymphocytes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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