Abstract
Microglia cells are the principal immune effector elements of the brain responding to any pathological event. To elucidate the possible role of microglia in initial non-inflammatory demyelination in canine distemper virus (CDV) infection, microglia from experimentally CDV infected dogs were isolated ex vivo by density gradient centrifugation and characterized immunophenotypically and functionally using flow cytometry. Results from dogs with demyelinating lesions were compared to results from recovered dogs and two healthy controls. CDV antigen could be detected in microglia of dogs with histopathologically confirmed demyelination. Microglia of these dogs showed marked upregulation of the surface molecules CD18, CD11b, CD11c, CD1c, MHC class I and MHC class II and a tendency for increased expression intensity of ICAM-1 (CD54), B7-1 (CD80), B7-2 (CD86), whereas no increased expression was found for CD44 and CD45. Functionally, microglia exhibited distinctly enhanced phagocytosis and generation of reactive oxygen species (ROS). It was concluded that in CDV infection, there is a clear association between microglial activation and demyelination. This strongly suggests that microglia contribute to acute myelin destruction in distemper.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 122-131 |
| Number of pages | 10 |
| Journal | Journal of Neuroimmunology |
| Volume | 153 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Aug 2004 |
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Keywords
- Canine distemper
- Demyelination
- Dog
- Ex vivo functional examination
- Ex vivo immunophenotypical examination
- Microglia
ASJC Scopus subject areas
- Immunology
- Clinical Neurology
- Immunology and Allergy
- Neurology
Cite this
Microglial cell activation in demyelinating canine distemper lesions. / Stein, Veronika M.; Czub, Markus; Schreiner, Nicole; Moore, Peter F; Vandevelde, Marc; Zurbriggen, Andreas; Tipold, Andrea.
In: Journal of Neuroimmunology, Vol. 153, No. 1-2, 08.2004, p. 122-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microglial cell activation in demyelinating canine distemper lesions
AU - Stein, Veronika M.
AU - Czub, Markus
AU - Schreiner, Nicole
AU - Moore, Peter F
AU - Vandevelde, Marc
AU - Zurbriggen, Andreas
AU - Tipold, Andrea
PY - 2004/8
Y1 - 2004/8
N2 - Microglia cells are the principal immune effector elements of the brain responding to any pathological event. To elucidate the possible role of microglia in initial non-inflammatory demyelination in canine distemper virus (CDV) infection, microglia from experimentally CDV infected dogs were isolated ex vivo by density gradient centrifugation and characterized immunophenotypically and functionally using flow cytometry. Results from dogs with demyelinating lesions were compared to results from recovered dogs and two healthy controls. CDV antigen could be detected in microglia of dogs with histopathologically confirmed demyelination. Microglia of these dogs showed marked upregulation of the surface molecules CD18, CD11b, CD11c, CD1c, MHC class I and MHC class II and a tendency for increased expression intensity of ICAM-1 (CD54), B7-1 (CD80), B7-2 (CD86), whereas no increased expression was found for CD44 and CD45. Functionally, microglia exhibited distinctly enhanced phagocytosis and generation of reactive oxygen species (ROS). It was concluded that in CDV infection, there is a clear association between microglial activation and demyelination. This strongly suggests that microglia contribute to acute myelin destruction in distemper.
AB - Microglia cells are the principal immune effector elements of the brain responding to any pathological event. To elucidate the possible role of microglia in initial non-inflammatory demyelination in canine distemper virus (CDV) infection, microglia from experimentally CDV infected dogs were isolated ex vivo by density gradient centrifugation and characterized immunophenotypically and functionally using flow cytometry. Results from dogs with demyelinating lesions were compared to results from recovered dogs and two healthy controls. CDV antigen could be detected in microglia of dogs with histopathologically confirmed demyelination. Microglia of these dogs showed marked upregulation of the surface molecules CD18, CD11b, CD11c, CD1c, MHC class I and MHC class II and a tendency for increased expression intensity of ICAM-1 (CD54), B7-1 (CD80), B7-2 (CD86), whereas no increased expression was found for CD44 and CD45. Functionally, microglia exhibited distinctly enhanced phagocytosis and generation of reactive oxygen species (ROS). It was concluded that in CDV infection, there is a clear association between microglial activation and demyelination. This strongly suggests that microglia contribute to acute myelin destruction in distemper.
KW - Canine distemper
KW - Demyelination
KW - Dog
KW - Ex vivo functional examination
KW - Ex vivo immunophenotypical examination
KW - Microglia
UR - http://www.scopus.com/inward/record.url?scp=3242658280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3242658280&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2004.05.001
DO - 10.1016/j.jneuroim.2004.05.001
M3 - Article
C2 - 15265670
AN - SCOPUS:3242658280
VL - 153
SP - 122
EP - 131
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -