Microglial cell activation in demyelinating canine distemper lesions

Veronika M. Stein, Markus Czub, Nicole Schreiner, Peter F Moore, Marc Vandevelde, Andreas Zurbriggen, Andrea Tipold

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Microglia cells are the principal immune effector elements of the brain responding to any pathological event. To elucidate the possible role of microglia in initial non-inflammatory demyelination in canine distemper virus (CDV) infection, microglia from experimentally CDV infected dogs were isolated ex vivo by density gradient centrifugation and characterized immunophenotypically and functionally using flow cytometry. Results from dogs with demyelinating lesions were compared to results from recovered dogs and two healthy controls. CDV antigen could be detected in microglia of dogs with histopathologically confirmed demyelination. Microglia of these dogs showed marked upregulation of the surface molecules CD18, CD11b, CD11c, CD1c, MHC class I and MHC class II and a tendency for increased expression intensity of ICAM-1 (CD54), B7-1 (CD80), B7-2 (CD86), whereas no increased expression was found for CD44 and CD45. Functionally, microglia exhibited distinctly enhanced phagocytosis and generation of reactive oxygen species (ROS). It was concluded that in CDV infection, there is a clear association between microglial activation and demyelination. This strongly suggests that microglia contribute to acute myelin destruction in distemper.

Original languageEnglish (US)
Pages (from-to)122-131
Number of pages10
JournalJournal of Neuroimmunology
Volume153
Issue number1-2
DOIs
StatePublished - Aug 2004

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Keywords

  • Canine distemper
  • Demyelination
  • Dog
  • Ex vivo functional examination
  • Ex vivo immunophenotypical examination
  • Microglia

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Stein, V. M., Czub, M., Schreiner, N., Moore, P. F., Vandevelde, M., Zurbriggen, A., & Tipold, A. (2004). Microglial cell activation in demyelinating canine distemper lesions. Journal of Neuroimmunology, 153(1-2), 122-131. https://doi.org/10.1016/j.jneuroim.2004.05.001