Microfluidics-enabled combinatorial peptide library for high throughput screening

S. Zhao, Z. Bai, Kit Lam, Tingrui Pan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

As an enabling tool, combinatorial peptide library method has played a significant role in many areas of chemical and biomedical research. The existing combinatorial peptide library methods, such as the one-bead-one-compound library and the microarray-based libraries, allow either large scale synthesis and screening or easy peptide identification, but not both. In this paper, we develop a novel microfluidics-enabled combinatorial peptide library method to address high throughput large scale library synthesis, parallel screening and direct peptide identification as a whole. The applicability of our method is demonstrated by the synthesis and screening of a random a4b1 integrin-specific library with 65,536 permutations.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1506-1508
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Microfluidics
Peptides
Screening
Throughput
Microarrays

Keywords

  • A4b1 integrin
  • Combinatorial library
  • High throughput
  • Microfluidics
  • Peptide

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Zhao, S., Bai, Z., Lam, K., & Pan, T. (2014). Microfluidics-enabled combinatorial peptide library for high throughput screening. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1506-1508). Chemical and Biological Microsystems Society.

Microfluidics-enabled combinatorial peptide library for high throughput screening. / Zhao, S.; Bai, Z.; Lam, Kit; Pan, Tingrui.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1506-1508.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhao, S, Bai, Z, Lam, K & Pan, T 2014, Microfluidics-enabled combinatorial peptide library for high throughput screening. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 1506-1508, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Zhao S, Bai Z, Lam K, Pan T. Microfluidics-enabled combinatorial peptide library for high throughput screening. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1506-1508
Zhao, S. ; Bai, Z. ; Lam, Kit ; Pan, Tingrui. / Microfluidics-enabled combinatorial peptide library for high throughput screening. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1506-1508
@inproceedings{115e33dd48474445a530fcebe8f8ea9b,
title = "Microfluidics-enabled combinatorial peptide library for high throughput screening",
abstract = "As an enabling tool, combinatorial peptide library method has played a significant role in many areas of chemical and biomedical research. The existing combinatorial peptide library methods, such as the one-bead-one-compound library and the microarray-based libraries, allow either large scale synthesis and screening or easy peptide identification, but not both. In this paper, we develop a novel microfluidics-enabled combinatorial peptide library method to address high throughput large scale library synthesis, parallel screening and direct peptide identification as a whole. The applicability of our method is demonstrated by the synthesis and screening of a random a4b1 integrin-specific library with 65,536 permutations.",
keywords = "A4b1 integrin, Combinatorial library, High throughput, Microfluidics, Peptide",
author = "S. Zhao and Z. Bai and Kit Lam and Tingrui Pan",
year = "2014",
language = "English (US)",
isbn = "9780979806476",
pages = "1506--1508",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Microfluidics-enabled combinatorial peptide library for high throughput screening

AU - Zhao, S.

AU - Bai, Z.

AU - Lam, Kit

AU - Pan, Tingrui

PY - 2014

Y1 - 2014

N2 - As an enabling tool, combinatorial peptide library method has played a significant role in many areas of chemical and biomedical research. The existing combinatorial peptide library methods, such as the one-bead-one-compound library and the microarray-based libraries, allow either large scale synthesis and screening or easy peptide identification, but not both. In this paper, we develop a novel microfluidics-enabled combinatorial peptide library method to address high throughput large scale library synthesis, parallel screening and direct peptide identification as a whole. The applicability of our method is demonstrated by the synthesis and screening of a random a4b1 integrin-specific library with 65,536 permutations.

AB - As an enabling tool, combinatorial peptide library method has played a significant role in many areas of chemical and biomedical research. The existing combinatorial peptide library methods, such as the one-bead-one-compound library and the microarray-based libraries, allow either large scale synthesis and screening or easy peptide identification, but not both. In this paper, we develop a novel microfluidics-enabled combinatorial peptide library method to address high throughput large scale library synthesis, parallel screening and direct peptide identification as a whole. The applicability of our method is demonstrated by the synthesis and screening of a random a4b1 integrin-specific library with 65,536 permutations.

KW - A4b1 integrin

KW - Combinatorial library

KW - High throughput

KW - Microfluidics

KW - Peptide

UR - http://www.scopus.com/inward/record.url?scp=84941695849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941695849&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84941695849

SN - 9780979806476

SP - 1506

EP - 1508

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

ER -