Microfluidics-enabled combinatorial peptide library for high throughput screening

S. Zhao, Z. Bai, Kit Lam, Tingrui Pan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

As an enabling tool, combinatorial peptide library method has played a significant role in many areas of chemical and biomedical research. The existing combinatorial peptide library methods, such as the one-bead-one-compound library and the microarray-based libraries, allow either large scale synthesis and screening or easy peptide identification, but not both. In this paper, we develop a novel microfluidics-enabled combinatorial peptide library method to address high throughput large scale library synthesis, parallel screening and direct peptide identification as a whole. The applicability of our method is demonstrated by the synthesis and screening of a random a4b1 integrin-specific library with 65,536 permutations.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1506-1508
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • A4b1 integrin
  • Combinatorial library
  • High throughput
  • Microfluidics
  • Peptide

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Zhao, S., Bai, Z., Lam, K., & Pan, T. (2014). Microfluidics-enabled combinatorial peptide library for high throughput screening. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1506-1508). Chemical and Biological Microsystems Society.