Microfluidic print-to-synthesis enabled combinatorial peptide microarray for cancer targeting

J. Li, Randy Carney, Ruiwu Liu, J. Fan, S. Zhao, Kit Lam, Tingrui Pan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This paper reports our newly developed microfluidic print-to-screen (MPS) platform and its application for synthesizing combinatorial peptide on PEG microdisc array. The MPS platform possesses several distinct features including easy operation, free of contamination, multiplexibility, low cost, high throughput and array density. The performance of the MPS system is characterized and, as proof of concept, a tetrapeptide library was constructed with an array density of 2560 spots/slide using the MPS approach. The library was screened for ligands targeting α4β1 integrin overexpressed on tumor cell plasma membranes. Repeatable outcomes demonstrate the feasibility of the MPS platform, as well as identification of positive sequences that actively bind to tumor cells.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages2195-2198
Number of pages4
ISBN (Electronic)9781538627310
DOIs
StatePublished - Jul 26 2017
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: Jun 18 2017Jun 22 2017

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period6/18/176/22/17

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Keywords

  • cancer targeting
  • combinatorial peptide library
  • microarray
  • Microfluidic printing
  • PEG

ASJC Scopus subject areas

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Li, J., Carney, R., Liu, R., Fan, J., Zhao, S., Lam, K., & Pan, T. (2017). Microfluidic print-to-synthesis enabled combinatorial peptide microarray for cancer targeting. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 2195-2198). [7994512] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994512