Microfluidic platform to examine tumor angiogenesis and metastasis at high spatiotemporal resolution

Venktesh S. Shirure; Steven George

Microfluidic platform to examine tumor angiogenesis and metastasis at high spatiotemporal resolution

Venktesh S. Shirure, Steven George

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The process of cancer metastasis follows a complex cascade of events. Animal models, the conventional model of choice, have improved our understanding of metastasis, but they provide limited spatial and temporal resolution of cellular events, and are thus not suitable to understand microenvironmental stimuli. Using microtechnology and tissue engineering, we have developed a microfluidic platform that supports growth of tumor spheroids spatially separated, but in communication through controlled diffusion and convection, from vascular networks. In this study we have characterized interstitial flow patterns and morphogen distribution within the platform, and then demonstrated its utility by assessing the effect of interstitial flow and vascular endothelial growth factor (VEGF) gradients on angiogenesis. We found that vessel sprouting is independently influenced by interstitial flow and the VEGF spatial gradient.

Original language	English (US)
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	181-183
Number of pages	3
ISBN (Electronic)	9780979806476
State	Published - Jan 1 2014
Externally published	Yes
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country	United States
City	San Antonio
Period	10/26/14 → 10/30/14

Keywords

Angiogenesis
Cancer technologies
Organ-on-a-chip
Tumor microenvironment

ASJC Scopus subject areas

Control and Systems Engineering

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Microfluidic platform to examine tumor angiogenesis and metastasis at high spatiotemporal resolution. / Shirure, Venktesh S.; George, Steven.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 181-183.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shirure, VS & George, S 2014, Microfluidic platform to examine tumor angiogenesis and metastasis at high spatiotemporal resolution. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 181-183, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.

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