Microfluidic platform to examine tumor angiogenesis and metastasis at high spatiotemporal resolution

Venktesh S. Shirure, Steven George

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The process of cancer metastasis follows a complex cascade of events. Animal models, the conventional model of choice, have improved our understanding of metastasis, but they provide limited spatial and temporal resolution of cellular events, and are thus not suitable to understand microenvironmental stimuli. Using microtechnology and tissue engineering, we have developed a microfluidic platform that supports growth of tumor spheroids spatially separated, but in communication through controlled diffusion and convection, from vascular networks. In this study we have characterized interstitial flow patterns and morphogen distribution within the platform, and then demonstrated its utility by assessing the effect of interstitial flow and vascular endothelial growth factor (VEGF) gradients on angiogenesis. We found that vessel sprouting is independently influenced by interstitial flow and the VEGF spatial gradient.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages181-183
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Angiogenesis
  • Cancer technologies
  • Organ-on-a-chip
  • Tumor microenvironment

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Shirure, V. S., & George, S. (2014). Microfluidic platform to examine tumor angiogenesis and metastasis at high spatiotemporal resolution. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 181-183). Chemical and Biological Microsystems Society.