Microfluidic human genetic profiling using fully non-contact temperature control

Daniel C. Leslie; Lindsay A. Legendre; Erkin Seker; Briony C. Strachan; James P. Landers

Microfluidic human genetic profiling using fully non-contact temperature control

Daniel C. Leslie, Lindsay A. Legendre, Erkin Seker, Briony C. Strachan, James P. Landers

Research output: Contribution to conference › Paper › peer-review

Abstract

Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.

Original language	English (US)
Pages	1731-1733
Number of pages	3
State	Published - Jan 1 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Genetic profiling
Interferometry
Multiplex
PCR
Temperature

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

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Microfluidic human genetic profiling using fully non-contact temperature control. / Leslie, Daniel C.; Legendre, Lindsay A.; Seker, Erkin; Strachan, Briony C.; Landers, James P.

2008. 1731-1733 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.",

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AU - Leslie, Daniel C.

AU - Legendre, Lindsay A.

AU - Seker, Erkin

AU - Strachan, Briony C.

AU - Landers, James P.

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AB - Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.

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KW - Interferometry

KW - Multiplex

KW - PCR

KW - Temperature

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