Microfluidic human genetic profiling using fully non-contact temperature control

Daniel C. Leslie, Lindsay A. Legendre, Erkin Seker, Briony C. Strachan, James P. Landers

Research output: Contribution to conferencePaper

Abstract

Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.

Original languageEnglish (US)
Pages1731-1733
Number of pages3
StatePublished - Jan 1 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Fingerprint

Polymerase chain reaction
Microfluidics
Temperature control
Microsatellite Repeats
Infrared heating
Bacteriophages
Thermocouples
Interferometers
Amplification
DNA
Genes
Calibration
Cooling
Temperature

Keywords

  • Genetic profiling
  • Interferometry
  • Multiplex
  • PCR
  • Temperature

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Leslie, D. C., Legendre, L. A., Seker, E., Strachan, B. C., & Landers, J. P. (2008). Microfluidic human genetic profiling using fully non-contact temperature control. 1731-1733. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Microfluidic human genetic profiling using fully non-contact temperature control. / Leslie, Daniel C.; Legendre, Lindsay A.; Seker, Erkin; Strachan, Briony C.; Landers, James P.

2008. 1731-1733 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conferencePaper

Leslie, DC, Legendre, LA, Seker, E, Strachan, BC & Landers, JP 2008, 'Microfluidic human genetic profiling using fully non-contact temperature control' Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08 - 10/16/08, pp. 1731-1733.
Leslie DC, Legendre LA, Seker E, Strachan BC, Landers JP. Microfluidic human genetic profiling using fully non-contact temperature control. 2008. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.
Leslie, Daniel C. ; Legendre, Lindsay A. ; Seker, Erkin ; Strachan, Briony C. ; Landers, James P. / Microfluidic human genetic profiling using fully non-contact temperature control. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.3 p.
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