Abstract
Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.
| Original language | English (US) |
|---|---|
| Pages | 1731-1733 |
| Number of pages | 3 |
| State | Published - Jan 1 2008 |
| Externally published | Yes |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008 |
Other
| Other | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| Country | United States |
| City | San Diego, CA |
| Period | 10/12/08 → 10/16/08 |
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Keywords
- Genetic profiling
- Interferometry
- Multiplex
- PCR
- Temperature
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Microfluidic human genetic profiling using fully non-contact temperature control. / Leslie, Daniel C.; Legendre, Lindsay A.; Seker, Erkin; Strachan, Briony C.; Landers, James P.
2008. 1731-1733 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.Research output: Contribution to conference › Paper
}
TY - CONF
T1 - Microfluidic human genetic profiling using fully non-contact temperature control
AU - Leslie, Daniel C.
AU - Legendre, Lindsay A.
AU - Seker, Erkin
AU - Strachan, Briony C.
AU - Landers, James P.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.
AB - Polymerase chain reaction (PCR) amplification of short tandem repeats (STR) present in the human genome is critical for forensic human identification. Presented here is the first report of non-contact temperature control for human genetic profiling. The non-contact temperature control consists of IR-mediated heating, convective cooling, and interferometic temperature sensing. The interferometer is originally calibrated with a miniature thermocouple in the PCR chamber resulting in a temperature sensitivity of 24.1 nm/°C. The calibration is successfully used to amplify a region of λ-phage DNA. Ultimately, 9 STR loci are successfully amplified for human identification.
KW - Genetic profiling
KW - Interferometry
KW - Multiplex
KW - PCR
KW - Temperature
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UR - http://www.scopus.com/inward/citedby.url?scp=84902532334&partnerID=8YFLogxK
M3 - Paper
SP - 1731
EP - 1733
ER -