Abstract
Models that aim to recapitulate the dynamic in vivo features of the microcirculation are crucial for studying vascularization. Cells in vivo respond not only to biochemical cues (e.g., growth factor gradients) but also sense mechanical cues (e.g., interstitial flow, vessel perfusion). Integrating the response of cells, the stroma, and the circulation in a dynamic 3D setting will create an environment suitable for the exploration of many fundamental vascularization processes. Here in this chapter, we describe an in vivo-inspired microenvironment that is conducive to the development of perfused human capillaries.
| Original language | English (US) |
|---|---|
| Title of host publication | Biomimetics and Stem Cells |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 21-27 |
| Number of pages | 7 |
| ISBN (Print) | 9781493913312 |
| DOIs | |
| State | Published - Jan 1 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1202 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Microenviroment
- Microfluidic platform
- Perfused capillary networks
- Tissue engineering
- Vasculogenesis
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Microfluidic device to culture 3D in vitro human capillary networks. / Moya, Monica L.; Alonzo, Luis F.; George, Steven.
Biomimetics and Stem Cells: Methods and Protocols. Humana Press Inc., 2014. p. 21-27 (Methods in Molecular Biology; Vol. 1202).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Microfluidic device to culture 3D in vitro human capillary networks
AU - Moya, Monica L.
AU - Alonzo, Luis F.
AU - George, Steven
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Models that aim to recapitulate the dynamic in vivo features of the microcirculation are crucial for studying vascularization. Cells in vivo respond not only to biochemical cues (e.g., growth factor gradients) but also sense mechanical cues (e.g., interstitial flow, vessel perfusion). Integrating the response of cells, the stroma, and the circulation in a dynamic 3D setting will create an environment suitable for the exploration of many fundamental vascularization processes. Here in this chapter, we describe an in vivo-inspired microenvironment that is conducive to the development of perfused human capillaries.
AB - Models that aim to recapitulate the dynamic in vivo features of the microcirculation are crucial for studying vascularization. Cells in vivo respond not only to biochemical cues (e.g., growth factor gradients) but also sense mechanical cues (e.g., interstitial flow, vessel perfusion). Integrating the response of cells, the stroma, and the circulation in a dynamic 3D setting will create an environment suitable for the exploration of many fundamental vascularization processes. Here in this chapter, we describe an in vivo-inspired microenvironment that is conducive to the development of perfused human capillaries.
KW - Microenviroment
KW - Microfluidic platform
KW - Perfused capillary networks
KW - Tissue engineering
KW - Vasculogenesis
UR - http://www.scopus.com/inward/record.url?scp=84907303250&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907303250&partnerID=8YFLogxK
U2 - 10.1007/7651-2013-36
DO - 10.1007/7651-2013-36
M3 - Chapter
C2 - 24155229
AN - SCOPUS:84907303250
SN - 9781493913312
T3 - Methods in Molecular Biology
SP - 21
EP - 27
BT - Biomimetics and Stem Cells
PB - Humana Press Inc.
ER -