Microdissection of peripheral nerve. Collagen and lipid distribution with morphological correlation

Mark J. Brown, David E Pleasure, Arthur K. Asbury

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Peripheral nerve is a complex tissue composed of endoneurial fascicles surrounded by perineurium and epineurium. We separated endoneurium from peri- and epineurium in human sural nerves by "endoneurial plucking", a method of microdissection. Endoneurial contents (axons, myelin sheaths, Schwann cells, vessels, and interstitial collagen) were cleanly separated in high yield from enveloping connective tissue, by both microscopic and biochemical criteria. Most of the nerve sulfatide and unesterified sterol was found in the endoneurial fraction while most of the collagen was in the peri-epineurial fraction. This microdissection method should prove useful in biochemical investigations of peripheral nerve.

Original languageEnglish (US)
Pages (from-to)361-369
Number of pages9
JournalJournal of the Neurological Sciences
Volume29
Issue number2-4
DOIs
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

Fingerprint Dive into the research topics of 'Microdissection of peripheral nerve. Collagen and lipid distribution with morphological correlation'. Together they form a unique fingerprint.

  • Cite this