Abstract
Peripheral nerve is a complex tissue composed of endoneurial fascicles surrounded by perineurium and epineurium. We separated endoneurium from peri- and epineurium in human sural nerves by "endoneurial plucking", a method of microdissection. Endoneurial contents (axons, myelin sheaths, Schwann cells, vessels, and interstitial collagen) were cleanly separated in high yield from enveloping connective tissue, by both microscopic and biochemical criteria. Most of the nerve sulfatide and unesterified sterol was found in the endoneurial fraction while most of the collagen was in the peri-epineurial fraction. This microdissection method should prove useful in biochemical investigations of peripheral nerve.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 361-369 |
| Number of pages | 9 |
| Journal | Journal of the Neurological Sciences |
| Volume | 29 |
| Issue number | 2-4 |
| DOIs | |
| State | Published - 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Aging
- Clinical Neurology
- Surgery
- Neuroscience(all)
- Developmental Neuroscience
- Neurology
Cite this
Microdissection of peripheral nerve. Collagen and lipid distribution with morphological correlation. / Brown, Mark J.; Pleasure, David E; Asbury, Arthur K.
In: Journal of the Neurological Sciences, Vol. 29, No. 2-4, 1976, p. 361-369.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microdissection of peripheral nerve. Collagen and lipid distribution with morphological correlation
AU - Brown, Mark J.
AU - Pleasure, David E
AU - Asbury, Arthur K.
PY - 1976
Y1 - 1976
N2 - Peripheral nerve is a complex tissue composed of endoneurial fascicles surrounded by perineurium and epineurium. We separated endoneurium from peri- and epineurium in human sural nerves by "endoneurial plucking", a method of microdissection. Endoneurial contents (axons, myelin sheaths, Schwann cells, vessels, and interstitial collagen) were cleanly separated in high yield from enveloping connective tissue, by both microscopic and biochemical criteria. Most of the nerve sulfatide and unesterified sterol was found in the endoneurial fraction while most of the collagen was in the peri-epineurial fraction. This microdissection method should prove useful in biochemical investigations of peripheral nerve.
AB - Peripheral nerve is a complex tissue composed of endoneurial fascicles surrounded by perineurium and epineurium. We separated endoneurium from peri- and epineurium in human sural nerves by "endoneurial plucking", a method of microdissection. Endoneurial contents (axons, myelin sheaths, Schwann cells, vessels, and interstitial collagen) were cleanly separated in high yield from enveloping connective tissue, by both microscopic and biochemical criteria. Most of the nerve sulfatide and unesterified sterol was found in the endoneurial fraction while most of the collagen was in the peri-epineurial fraction. This microdissection method should prove useful in biochemical investigations of peripheral nerve.
UR - http://www.scopus.com/inward/record.url?scp=0017192595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017192595&partnerID=8YFLogxK
U2 - 10.1016/0022-510X(76)90185-4
DO - 10.1016/0022-510X(76)90185-4
M3 - Article
C2 - 978216
AN - SCOPUS:0017192595
VL - 29
SP - 361
EP - 369
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 2-4
ER -