Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via toll-like receptor 2 activation in the intestinal mucosa

Jessalyn H. Nishimori, Tiffanny N. Newman, Gertrude O. Oppong, Glenn J. Rapsinski, Jui Hung Yen, Steven G. Biesecker, R. Paul Wilson, Brian P. Butler, Maria G. Winter, Renee M Tsolis, Doina Ganea, çagla Tükela

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, we investigated whether expression of curli amyloid fibrils by the invasive enteric pathogen Salmonella enterica serotype Typhimurium contributes to T helper 1 and T helper 17 responses by measuring cytokine production in the mouse colitis model. A csgBA mutant, deficient in curli production, elicited decreased expression of interleukin 17A (IL-17A) and IL-22 in the cecal mucosa compared to the S. Typhimurium wild type. In TLR2-deficient mice, IL-17A and IL-22 expression was blunted during S. Typhimurium infection, suggesting that activation of the TLR2 signaling pathway contributes to the expression of these cytokines. T cells incubated with supernatants from bone marrow-derived dendritic cells (BMDCs) treated with curli fibrils released IL-17A in a TLR2-dependent manner in vitro. Lower levels of IL-6 and IL-23 production were detected in the supernatants of the TLR2-deficient BMDCs treated with curli fibrils. Consistent with this, three distinct T-cell populations-CD4+ T helper cells, cytotoxic CD8+ T cells, and γδ T cells-produced IL-17A in response to curli fibrils in the intestinal mucosa during S. Typhimurium infection. Notably, decreased IL-6 expression by the dendritic cells and decreased IL-23 expression by the dendritic cells and macrophages were observed in the cecal mucosa of mice infected with the curli mutant. We conclude that TLR2 recognition of bacterial amyloid fibrils in the intestinal mucosa represents a novel mechanism of immunoregulation, which contributes to the generation ofinflammatory responses, including production of IL-17A and IL-22, in response to bacterial entry into the intestinal mucosa.

Original languageEnglish (US)
Pages (from-to)4398-4408
Number of pages11
JournalInfection and Immunity
Volume80
Issue number12
DOIs
StatePublished - 2012

Fingerprint

Toll-Like Receptor 2
Interleukin-17
Intestinal Mucosa
Amyloid
Dendritic Cells
Interleukin-23
T-Lymphocytes
Interleukin-6
Mucous Membrane
Toll-Like Receptor 1
Bone Marrow
Bacteroidetes
Cytokines
Proteobacteria
Salmonella enterica
Colitis
Biofilms
Helper-Inducer T-Lymphocytes
Infection
interleukin-22

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Nishimori, J. H., Newman, T. N., Oppong, G. O., Rapsinski, G. J., Yen, J. H., Biesecker, S. G., ... Tükela, Ç. (2012). Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via toll-like receptor 2 activation in the intestinal mucosa. Infection and Immunity, 80(12), 4398-4408. https://doi.org/10.1128/IAI.00911-12

Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via toll-like receptor 2 activation in the intestinal mucosa. / Nishimori, Jessalyn H.; Newman, Tiffanny N.; Oppong, Gertrude O.; Rapsinski, Glenn J.; Yen, Jui Hung; Biesecker, Steven G.; Wilson, R. Paul; Butler, Brian P.; Winter, Maria G.; Tsolis, Renee M; Ganea, Doina; Tükela, çagla.

In: Infection and Immunity, Vol. 80, No. 12, 2012, p. 4398-4408.

Research output: Contribution to journalArticle

Nishimori, JH, Newman, TN, Oppong, GO, Rapsinski, GJ, Yen, JH, Biesecker, SG, Wilson, RP, Butler, BP, Winter, MG, Tsolis, RM, Ganea, D & Tükela, Ç 2012, 'Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via toll-like receptor 2 activation in the intestinal mucosa', Infection and Immunity, vol. 80, no. 12, pp. 4398-4408. https://doi.org/10.1128/IAI.00911-12
Nishimori, Jessalyn H. ; Newman, Tiffanny N. ; Oppong, Gertrude O. ; Rapsinski, Glenn J. ; Yen, Jui Hung ; Biesecker, Steven G. ; Wilson, R. Paul ; Butler, Brian P. ; Winter, Maria G. ; Tsolis, Renee M ; Ganea, Doina ; Tükela, çagla. / Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via toll-like receptor 2 activation in the intestinal mucosa. In: Infection and Immunity. 2012 ; Vol. 80, No. 12. pp. 4398-4408.
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