Microarray immunoassay for phenoxybenzoic acid using polymer-functionalized lanthanide oxide nanoparticles as fluorescent labels

Mikaela Nichkova, Dosi Dosev, Shirley J. Gee, Bruce D. Hammock, Ian M. Kennedy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Fluorescent properties and low production cost makes lanthanide oxide nanoparticles attractive labels in biochemistry. Nanoparticles with different fluorescent spectra were produced by doping of oxides such as Y 2O 3 and Gd 2O 3 with different lanthanide ions (Eu, Tb, Sm) giving the possibility for multicolor labeling. Protein microarrays have the potential to play a fundamental role in the miniaturization of biosensors, clinical immunological assays, and protein-protein interaction studies. Here we present the application of fluorescent lanthanide oxide nanoparticles as labels in microarray-based immunoassay for phenoxybenzoic acid (PBA), a generic biomarker of human exposure to the highly potent insecticides pyrethroids. A novel polymer-based protocol was developed for biochemical functionalization of the nanoparticles. Microarrays of antibodies were fabricated by microcontact printing in line patterns onto glass substrates and immunoassays were successfully performed using the corresponding functionalized nanoparticles. The applicability of the fluorophore nanoparticles as reporters for detection of antibody-antigen interactions has been demonstrated for phenoxybenzoic acid (PBA)/anti-PBA IgG. The sensitivity of the competitive fluorescent immunoassay for PBA was similar to that of the corresponding ELISA.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsM.S. Islam, A.K. Dutta
Volume6008
DOIs
StatePublished - 2005
EventNanosensing: Materials and Devices II - Boston, MA, United States
Duration: Oct 23 2005Oct 26 2005

Other

OtherNanosensing: Materials and Devices II
CountryUnited States
CityBoston, MA
Period10/23/0510/26/05

Keywords

  • Immunoassay
  • Luminescence
  • Microarray
  • Nanoparticles

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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    Nichkova, M., Dosev, D., Gee, S. J., Hammock, B. D., & Kennedy, I. M. (2005). Microarray immunoassay for phenoxybenzoic acid using polymer-functionalized lanthanide oxide nanoparticles as fluorescent labels. In M. S. Islam, & A. K. Dutta (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6008). [600815] https://doi.org/10.1117/12.631032