Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression

Patricia J. Champine, Jacob Michaelson, Bart C Weimer, Danny R. Welch, Daryll B. DeWald

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We used Affymetrix microarrays to compare gene expression profiles of the metastatic parental breast cancer cell line MDA-MB-435 (435) and the non-metastatic daughter cell line created by the stable expression of the BReast cancer Metastasis Suppressor 1 (BRMS1) gene in 435 cells, MDA-MB-435-BRMS1 (435/BRMS1). Analysis of microarray data provided insight into some of the potential mechanisms by which BRMS1 inhibits tumor formation at secondary sites. Furthermore, due to the importance of the microenvironment, we also examined gene expression under different growth conditions (i.e., plus or minus serum). Expression of 565 genes was significantly (adjusted P-value <0.05) altered regardless of in vitro growth conditions. BRMS1 expression significantly increased multiple major histocompatability complex (MHC) genes and significantly decreased expression of several genes associated with protein localization and secretion. The pattern of gene expression associated with BRMS1 expression suggests that metastasis suppression may be mediated by enhanced immune recognition, altered transport, and/or secretion of metastasis-associated proteins.

Original languageEnglish (US)
Pages (from-to)551-565
Number of pages15
JournalClinical and Experimental Metastasis
Volume24
Issue number7
DOIs
StatePublished - Nov 2007
Externally publishedYes

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Keywords

  • Affymetrix
  • Breast cancer
  • BRMS1
  • Metastasis
  • MHC
  • Microarray

ASJC Scopus subject areas

  • Cancer Research

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