Microarray analysis of differentially expressed genes of primary tumors in the canine central nervous system

S. A M Thomson, E. Kennerly, N. Olby, J. R. Mickelson, D. E. Hoffmann, Peter J Dickinson, G. Gibson, M. Breen

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34 Scopus citations

Abstract

The pathophysiologic similarities of many human and canine cancers support the role of the domestic dog as a model for brain tumor research. Here we report the construction of a custom canine brain-specific cDNA microarray and the analysis of gene expression patterns of several different types of canine brain tumor. The microarray contained 4000 clones from a canine brain specific cDNA library including 2161 clones that matched known genes or expressed sequence tags (ESTs) and 25 cancer-related genes. Our study included 16 brain tumors (seven meningiomas, five glial tumors, two ependymomas, and two choroid plexus papillomas) from a variety of different dog breeds. We identified several genes previously found to be differentially expressed in human brain tumors. This suggests that human and canine brain tumors share a common pathogenesis. In addition, we also found differentially expressed genes unique to either meningiomas or the glial tumors. This report represents the first global gene expression analysis of different types of canine brain tumors by cDNA microarrays and might aid in the identification of potential candidate genes involved in tumor formation and progression.

Original languageEnglish (US)
Pages (from-to)550-558
Number of pages9
JournalVeterinary Pathology
Volume42
Issue number5
DOIs
StatePublished - Sep 2005

Keywords

  • Canine choroid plexus tumor
  • cDNA microarray
  • Ependymoma
  • Glial tumor
  • Meningioma

ASJC Scopus subject areas

  • veterinary(all)

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    Thomson, S. A. M., Kennerly, E., Olby, N., Mickelson, J. R., Hoffmann, D. E., Dickinson, P. J., Gibson, G., & Breen, M. (2005). Microarray analysis of differentially expressed genes of primary tumors in the canine central nervous system. Veterinary Pathology, 42(5), 550-558. https://doi.org/10.1354/vp.42-5-550