Abstract
Microarrays and related technologies have allowed investigators to ask biological questions in far greater detail than has previously been possible. Microarrays had a troubled beginning, but most of these problems resulted from the growing pains of this technology, which, like many new things, was initially more promise than delivery. Nevertheless, over the past few years, investigators have learned how to achieve optimal performance of technology, and now exciting discoveries are made using microarray-based research. Many of the advances have come from the realization that microarrays are not a magic tool but rather are like any other measurement device. Unless microarray experimentation is coupled with good experimental practices, it will not yield valid results or, worse yet, may lead to misleading results. In this chapter, we highlight some of the important steps that should be taken to successfully conduct a microarray study. These steps include a clearly stated biological question, experimental design, careful experimental conduct, complete statistical analysis, validation/verification of results, and dissemination of the data.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 409-430 |
| Number of pages | 22 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 404 |
| State | Published - 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Microarray analysis. / Page, Grier P.; Zakharkin, Stanislav O.; Kim, Kyoungmi; Mehta, Tapan; Chen, Lang; Zhang, Kui.
In: Methods in molecular biology (Clifton, N.J.), Vol. 404, 2007, p. 409-430.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microarray analysis.
AU - Page, Grier P.
AU - Zakharkin, Stanislav O.
AU - Kim, Kyoungmi
AU - Mehta, Tapan
AU - Chen, Lang
AU - Zhang, Kui
PY - 2007
Y1 - 2007
N2 - Microarrays and related technologies have allowed investigators to ask biological questions in far greater detail than has previously been possible. Microarrays had a troubled beginning, but most of these problems resulted from the growing pains of this technology, which, like many new things, was initially more promise than delivery. Nevertheless, over the past few years, investigators have learned how to achieve optimal performance of technology, and now exciting discoveries are made using microarray-based research. Many of the advances have come from the realization that microarrays are not a magic tool but rather are like any other measurement device. Unless microarray experimentation is coupled with good experimental practices, it will not yield valid results or, worse yet, may lead to misleading results. In this chapter, we highlight some of the important steps that should be taken to successfully conduct a microarray study. These steps include a clearly stated biological question, experimental design, careful experimental conduct, complete statistical analysis, validation/verification of results, and dissemination of the data.
AB - Microarrays and related technologies have allowed investigators to ask biological questions in far greater detail than has previously been possible. Microarrays had a troubled beginning, but most of these problems resulted from the growing pains of this technology, which, like many new things, was initially more promise than delivery. Nevertheless, over the past few years, investigators have learned how to achieve optimal performance of technology, and now exciting discoveries are made using microarray-based research. Many of the advances have come from the realization that microarrays are not a magic tool but rather are like any other measurement device. Unless microarray experimentation is coupled with good experimental practices, it will not yield valid results or, worse yet, may lead to misleading results. In this chapter, we highlight some of the important steps that should be taken to successfully conduct a microarray study. These steps include a clearly stated biological question, experimental design, careful experimental conduct, complete statistical analysis, validation/verification of results, and dissemination of the data.
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M3 - Article
C2 - 18450061
AN - SCOPUS:43749114264
VL - 404
SP - 409
EP - 430
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -