Microarray analysis.

Grier P. Page, Stanislav O. Zakharkin, Kyoungmi Kim, Tapan Mehta, Lang Chen, Kui Zhang

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Microarrays and related technologies have allowed investigators to ask biological questions in far greater detail than has previously been possible. Microarrays had a troubled beginning, but most of these problems resulted from the growing pains of this technology, which, like many new things, was initially more promise than delivery. Nevertheless, over the past few years, investigators have learned how to achieve optimal performance of technology, and now exciting discoveries are made using microarray-based research. Many of the advances have come from the realization that microarrays are not a magic tool but rather are like any other measurement device. Unless microarray experimentation is coupled with good experimental practices, it will not yield valid results or, worse yet, may lead to misleading results. In this chapter, we highlight some of the important steps that should be taken to successfully conduct a microarray study. These steps include a clearly stated biological question, experimental design, careful experimental conduct, complete statistical analysis, validation/verification of results, and dissemination of the data.

Original languageEnglish (US)
Pages (from-to)409-430
Number of pages22
JournalMethods in molecular biology (Clifton, N.J.)
Volume404
StatePublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Page, G. P., Zakharkin, S. O., Kim, K., Mehta, T., Chen, L., & Zhang, K. (2007). Microarray analysis. Methods in molecular biology (Clifton, N.J.), 404, 409-430.