Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors

José María Sansiñena, Chanel K. Yee, Annapoorna Sapuri, Basil Swanson, Antonio Redondo, Atul N. Parikh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a new approach for the design of ionic reservoir arrays within a double phospholipid bilayer to ultimately develop a 2D array of ion-channel switch based electrochemical biosensors. As a first step, a primary bilayer lipid membrane (BLM) is deposited onto an array of electrodes patterned onto a substrate surface. Subsequently, an array of microvoids is created within the bilayer by a wet photolithographic patterning of phospholipid bilayers using a deep UV light source and a quartz/chrome photomask. The deposition of a secondary BLM onto the primary bilayer that spans across the patterned microvoids leads to the formation of an array of ionic reservoirs within the double phospholipid bilayer. This is accomplished using giant unilamellar vesicles and by exploiting membrane electrostatics. The eventual use of ligand-gated ion-channels incorporated into the secondary bilayer that covers the individual ionic reservoirs will lead to a 2D array of ion-channel switch based electrochemical biosensors.

Original languageEnglish (US)
Title of host publication2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
EditorsM. Laudon, B. Romanowicz
Pages221-223
Number of pages3
Volume1
StatePublished - 2004
Event2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 - Boston, MA, United States
Duration: Mar 7 2004Mar 11 2004

Other

Other2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
CountryUnited States
CityBoston, MA
Period3/7/043/11/04

Fingerprint

Lipid bilayers
Phospholipids
Biosensors
Switches
Ions
Photomasks
Ultraviolet radiation
Quartz
Electrostatics
Ligands
Membranes
Electrodes
Substrates
Membrane Lipids

Keywords

  • Bilayer lipid membrane
  • Electrochemical biosensor
  • Ion channel
  • Ionic reservoir

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sansiñena, J. M., Yee, C. K., Sapuri, A., Swanson, B., Redondo, A., & Parikh, A. N. (2004). Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors. In M. Laudon, & B. Romanowicz (Eds.), 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 (Vol. 1, pp. 221-223)

Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors. / Sansiñena, José María; Yee, Chanel K.; Sapuri, Annapoorna; Swanson, Basil; Redondo, Antonio; Parikh, Atul N.

2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004. ed. / M. Laudon; B. Romanowicz. Vol. 1 2004. p. 221-223.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sansiñena, JM, Yee, CK, Sapuri, A, Swanson, B, Redondo, A & Parikh, AN 2004, Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors. in M Laudon & B Romanowicz (eds), 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004. vol. 1, pp. 221-223, 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004, Boston, MA, United States, 3/7/04.
Sansiñena JM, Yee CK, Sapuri A, Swanson B, Redondo A, Parikh AN. Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors. In Laudon M, Romanowicz B, editors, 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004. Vol. 1. 2004. p. 221-223
Sansiñena, José María ; Yee, Chanel K. ; Sapuri, Annapoorna ; Swanson, Basil ; Redondo, Antonio ; Parikh, Atul N. / Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors. 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004. editor / M. Laudon ; B. Romanowicz. Vol. 1 2004. pp. 221-223
@inproceedings{3db09ee02c1e4d91ad1682e565f190fe,
title = "Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors",
abstract = "We present a new approach for the design of ionic reservoir arrays within a double phospholipid bilayer to ultimately develop a 2D array of ion-channel switch based electrochemical biosensors. As a first step, a primary bilayer lipid membrane (BLM) is deposited onto an array of electrodes patterned onto a substrate surface. Subsequently, an array of microvoids is created within the bilayer by a wet photolithographic patterning of phospholipid bilayers using a deep UV light source and a quartz/chrome photomask. The deposition of a secondary BLM onto the primary bilayer that spans across the patterned microvoids leads to the formation of an array of ionic reservoirs within the double phospholipid bilayer. This is accomplished using giant unilamellar vesicles and by exploiting membrane electrostatics. The eventual use of ligand-gated ion-channels incorporated into the secondary bilayer that covers the individual ionic reservoirs will lead to a 2D array of ion-channel switch based electrochemical biosensors.",
keywords = "Bilayer lipid membrane, Electrochemical biosensor, Ion channel, Ionic reservoir",
author = "Sansi{\~n}ena, {Jos{\'e} Mar{\'i}a} and Yee, {Chanel K.} and Annapoorna Sapuri and Basil Swanson and Antonio Redondo and Parikh, {Atul N.}",
year = "2004",
language = "English (US)",
isbn = "0972842276",
volume = "1",
pages = "221--223",
editor = "M. Laudon and B. Romanowicz",
booktitle = "2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004",

}

TY - GEN

T1 - Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors

AU - Sansiñena, José María

AU - Yee, Chanel K.

AU - Sapuri, Annapoorna

AU - Swanson, Basil

AU - Redondo, Antonio

AU - Parikh, Atul N.

PY - 2004

Y1 - 2004

N2 - We present a new approach for the design of ionic reservoir arrays within a double phospholipid bilayer to ultimately develop a 2D array of ion-channel switch based electrochemical biosensors. As a first step, a primary bilayer lipid membrane (BLM) is deposited onto an array of electrodes patterned onto a substrate surface. Subsequently, an array of microvoids is created within the bilayer by a wet photolithographic patterning of phospholipid bilayers using a deep UV light source and a quartz/chrome photomask. The deposition of a secondary BLM onto the primary bilayer that spans across the patterned microvoids leads to the formation of an array of ionic reservoirs within the double phospholipid bilayer. This is accomplished using giant unilamellar vesicles and by exploiting membrane electrostatics. The eventual use of ligand-gated ion-channels incorporated into the secondary bilayer that covers the individual ionic reservoirs will lead to a 2D array of ion-channel switch based electrochemical biosensors.

AB - We present a new approach for the design of ionic reservoir arrays within a double phospholipid bilayer to ultimately develop a 2D array of ion-channel switch based electrochemical biosensors. As a first step, a primary bilayer lipid membrane (BLM) is deposited onto an array of electrodes patterned onto a substrate surface. Subsequently, an array of microvoids is created within the bilayer by a wet photolithographic patterning of phospholipid bilayers using a deep UV light source and a quartz/chrome photomask. The deposition of a secondary BLM onto the primary bilayer that spans across the patterned microvoids leads to the formation of an array of ionic reservoirs within the double phospholipid bilayer. This is accomplished using giant unilamellar vesicles and by exploiting membrane electrostatics. The eventual use of ligand-gated ion-channels incorporated into the secondary bilayer that covers the individual ionic reservoirs will lead to a 2D array of ion-channel switch based electrochemical biosensors.

KW - Bilayer lipid membrane

KW - Electrochemical biosensor

KW - Ion channel

KW - Ionic reservoir

UR - http://www.scopus.com/inward/record.url?scp=6344246191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6344246191&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:6344246191

SN - 0972842276

SN - 9780972842273

VL - 1

SP - 221

EP - 223

BT - 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004

A2 - Laudon, M.

A2 - Romanowicz, B.

ER -