Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors

José María Sansiñena, Chanel K. Yee, Annapoorna Sapuri, Basil Swanson, Antonio Redondo, Atul N. Parikh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We present a new approach for the design of ionic reservoir arrays within a double phospholipid bilayer to ultimately develop a 2D array of ion-channel switch based electrochemical biosensors. As a first step, a primary bilayer lipid membrane (BLM) is deposited onto an array of electrodes patterned onto a substrate surface. Subsequently, an array of microvoids is created within the bilayer by a wet photolithographic patterning of phospholipid bilayers using a deep UV light source and a quartz/chrome photomask. The deposition of a secondary BLM onto the primary bilayer that spans across the patterned microvoids leads to the formation of an array of ionic reservoirs within the double phospholipid bilayer. This is accomplished using giant unilamellar vesicles and by exploiting membrane electrostatics. The eventual use of ligand-gated ion-channels incorporated into the secondary bilayer that covers the individual ionic reservoirs will lead to a 2D array of ion-channel switch based electrochemical biosensors.

Original languageEnglish (US)
Title of host publication2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
EditorsM. Laudon, B. Romanowicz
Pages221-223
Number of pages3
Volume1
StatePublished - 2004
Event2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 - Boston, MA, United States
Duration: Mar 7 2004Mar 11 2004

Other

Other2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
CountryUnited States
CityBoston, MA
Period3/7/043/11/04

Keywords

  • Bilayer lipid membrane
  • Electrochemical biosensor
  • Ion channel
  • Ionic reservoir

ASJC Scopus subject areas

  • Engineering(all)

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    Sansiñena, J. M., Yee, C. K., Sapuri, A., Swanson, B., Redondo, A., & Parikh, A. N. (2004). Micro-patterning of ionic reservoirs within a double bilayer lipid membrane to fabricate a 2D array of ion-channel switch based electrochemical biosensors. In M. Laudon, & B. Romanowicz (Eds.), 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 (Vol. 1, pp. 221-223)