Micro-lipid-droplet encapsulation of Bacillus thuringiensis subsp. israelensis δ-endotoxin for control of mosquito larvae

P. Y K Cheung, B. D. Hammock

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The crystal δ-endotoxin of Bacillus thuringiensis subsp. israelensis is less toxic to larvae of Anopheles freeborni than to larvae of Aedes aegypti. However, when solubilized crystal was used, larvae from both species showed similar sensitivities. This effect presumably was due to the differences in feeding behavior between the two mosquito larvae when crystal preparations are used. A procedure is described whereby both crystal and solubilized B. thuringiensis subsp. israelensis toxin were emulsified with Freund incomplete adjuvant, with retention of toxicity. The use of Freund incomplete adjuvant also allowed one to assay the solubilized toxin at a low nanogram level. Furthermore, coating the toxin with lipophilic material altered the buoyancy of the toxin and reversed the sensitivities of the two mosquito larvae toward the B. thuringiensis subsp. israelensis toxin. This difference in buoyancy was determined by using an enzyme-linked immunosorbent assay that was specific for the toxic peptides. These data indicate that economically feasible buoyant formulations for the B. thuringiensis subsp. israelensis crystal can be developed.

Original languageEnglish (US)
Pages (from-to)984-988
Number of pages5
JournalApplied and Environmental Microbiology
Volume50
Issue number4
StatePublished - 1985

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

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