Micro-heterogeneity of flow in a mouse model of chronic cerebral hypoperfusion revealed by longitudinal Doppler optical coherence tomography and angiography

Vivek Srinivasan, Esther Yu, Harsha Radhakrishnan, Anil Can, Mihail Climov, Conor Leahy, Cenk Ayata, Katharina Eikermann-Haerter

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although microvascular dysfunction accompanies cognitive decline in aging, vascular dementia, and Alzheimer's disease, tools to study microvasculature longitudinally in vivo are lacking. Here, we use Doppler optical coherence tomography (OCT) and angiography for noninvasive, longitudinal imaging of mice with chronic cerebral hypoperfusion for up to 1 month. In particular, we optimized the OCT angiography method to selectively image red blood cell (RBC)-perfused capillaries, leading to a novel way of assessing capillary supply heterogeneity in vivo. After bilateral common carotid artery stenosis (BCAS), cortical blood flow measured by Doppler OCT dropped to half of baseline throughout the imaged tissue acutely. Microscopic imaging of the capillary bed with OCT angiography further revealed local heterogeneities in cortical flow supply during hypoperfusion. The number of RBC-perfused capillaries decreased, leading to increased oxygen diffusion distances in the days immediately after BCAS. Linear regression showed that RBC-perfused capillary density declined by 0.3% for a drop in flow of 1 mL/100 g per minute, and decreases in RBC-perfused capillary density as high as 25% were observed. Taken together, these results demonstrate the existence of local supply heterogeneity at the capillary level even at nonischemic global flow levels, and demonstrate a novel imaging method to assess this heterogeneity.

Original languageEnglish (US)
Pages (from-to)1552-1560
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume35
Issue number10
DOIs
StatePublished - Oct 3 2015

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Optical Coherence Tomography
Angiography
Erythrocytes
Carotid Stenosis
Vascular Dementia
Microvessels
Vascular Diseases
Linear Models
Alzheimer Disease
Oxygen

Keywords

  • angiography
  • capillary recruitment
  • Doppler optical coherence tomography
  • hypoperfusion
  • vascular dementia

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Micro-heterogeneity of flow in a mouse model of chronic cerebral hypoperfusion revealed by longitudinal Doppler optical coherence tomography and angiography. / Srinivasan, Vivek; Yu, Esther; Radhakrishnan, Harsha; Can, Anil; Climov, Mihail; Leahy, Conor; Ayata, Cenk; Eikermann-Haerter, Katharina.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 35, No. 10, 03.10.2015, p. 1552-1560.

Research output: Contribution to journalArticle

Srinivasan, Vivek ; Yu, Esther ; Radhakrishnan, Harsha ; Can, Anil ; Climov, Mihail ; Leahy, Conor ; Ayata, Cenk ; Eikermann-Haerter, Katharina. / Micro-heterogeneity of flow in a mouse model of chronic cerebral hypoperfusion revealed by longitudinal Doppler optical coherence tomography and angiography. In: Journal of Cerebral Blood Flow and Metabolism. 2015 ; Vol. 35, No. 10. pp. 1552-1560.
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