Mice with low levels of Shc proteins display reduced glycolytic and increased gluconeogenic activities in liver

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Abstract

Shc proteins play a role in energy metabolism through interaction with the insulin receptor. The aim of this study was to determine whether Shc proteins influence liver glycolysis and gluconeogenesis under both fed and fasted states. Decreased glycolytic and increased gluconeogenic and transamination enzyme activities were observed in ShcKO versus WT mice. Levels of key regulatory metabolites, such as fructose-2,6-bisphosphate, matched the activity of metabolic pathways. Protein levels of glycolytic and gluconeogenic enzymes were not different. pAMPK protein levels increased with fasting and were higher in ShcKO versus WT mice. Therefore, Shc proteins play a role in shifting the metabolism from glucose oxidation to gluconeogenesis and lipid catabolism and should be considered as regulators of fuel selection. Fuel selection and utilization could play a critical role in healthy aging. Characterization of metabolic events in ShcKO mice would help to elucidate how metabolism is influenced by these proteins.

Original languageEnglish (US)
Pages (from-to)273-286
Number of pages14
JournalBiochemistry and Biophysics Reports
Volume7
DOIs
StatePublished - Sep 1 2016

Fingerprint

Liver
Proteins
Gluconeogenesis
Metabolism
Insulin Receptor
Enzyme activity
Glycolysis
Enzymes
Metabolites
Metabolic Networks and Pathways
Energy Metabolism
Fasting
Aging of materials
Lipids
Glucose
Oxidation

Keywords

  • Fasting
  • Fuel selection
  • Gluconeogenesis
  • Glycolysis
  • Metabolism
  • Shc proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Mice with low levels of Shc proteins display reduced glycolytic and increased gluconeogenic activities in liver",
abstract = "Shc proteins play a role in energy metabolism through interaction with the insulin receptor. The aim of this study was to determine whether Shc proteins influence liver glycolysis and gluconeogenesis under both fed and fasted states. Decreased glycolytic and increased gluconeogenic and transamination enzyme activities were observed in ShcKO versus WT mice. Levels of key regulatory metabolites, such as fructose-2,6-bisphosphate, matched the activity of metabolic pathways. Protein levels of glycolytic and gluconeogenic enzymes were not different. pAMPK protein levels increased with fasting and were higher in ShcKO versus WT mice. Therefore, Shc proteins play a role in shifting the metabolism from glucose oxidation to gluconeogenesis and lipid catabolism and should be considered as regulators of fuel selection. Fuel selection and utilization could play a critical role in healthy aging. Characterization of metabolic events in ShcKO mice would help to elucidate how metabolism is influenced by these proteins.",
keywords = "Fasting, Fuel selection, Gluconeogenesis, Glycolysis, Metabolism, Shc proteins",
author = "Kevork Hagopian and Kyoungmi Kim and L{\'o}pez-Dominguez, {Jos{\'e} Alberto} and Alexey Tomilov and Cortopassi, {Gino A} and Ramsey, {Jon J}",
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T1 - Mice with low levels of Shc proteins display reduced glycolytic and increased gluconeogenic activities in liver

AU - Hagopian, Kevork

AU - Kim, Kyoungmi

AU - López-Dominguez, José Alberto

AU - Tomilov, Alexey

AU - Cortopassi, Gino A

AU - Ramsey, Jon J

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Shc proteins play a role in energy metabolism through interaction with the insulin receptor. The aim of this study was to determine whether Shc proteins influence liver glycolysis and gluconeogenesis under both fed and fasted states. Decreased glycolytic and increased gluconeogenic and transamination enzyme activities were observed in ShcKO versus WT mice. Levels of key regulatory metabolites, such as fructose-2,6-bisphosphate, matched the activity of metabolic pathways. Protein levels of glycolytic and gluconeogenic enzymes were not different. pAMPK protein levels increased with fasting and were higher in ShcKO versus WT mice. Therefore, Shc proteins play a role in shifting the metabolism from glucose oxidation to gluconeogenesis and lipid catabolism and should be considered as regulators of fuel selection. Fuel selection and utilization could play a critical role in healthy aging. Characterization of metabolic events in ShcKO mice would help to elucidate how metabolism is influenced by these proteins.

AB - Shc proteins play a role in energy metabolism through interaction with the insulin receptor. The aim of this study was to determine whether Shc proteins influence liver glycolysis and gluconeogenesis under both fed and fasted states. Decreased glycolytic and increased gluconeogenic and transamination enzyme activities were observed in ShcKO versus WT mice. Levels of key regulatory metabolites, such as fructose-2,6-bisphosphate, matched the activity of metabolic pathways. Protein levels of glycolytic and gluconeogenic enzymes were not different. pAMPK protein levels increased with fasting and were higher in ShcKO versus WT mice. Therefore, Shc proteins play a role in shifting the metabolism from glucose oxidation to gluconeogenesis and lipid catabolism and should be considered as regulators of fuel selection. Fuel selection and utilization could play a critical role in healthy aging. Characterization of metabolic events in ShcKO mice would help to elucidate how metabolism is influenced by these proteins.

KW - Fasting

KW - Fuel selection

KW - Gluconeogenesis

KW - Glycolysis

KW - Metabolism

KW - Shc proteins

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