MHC gene configuration variation in geographically disparate populations of California sea lions (Zalophus californianus)

L. Bowen, B. M. Aldridge, R. Delong, S. Melin, C. Godinez, A. Zavala, F. Gulland, Linda J Lowenstine, Jeffrey L Stott, M. L. Johnson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Major histocompatibility complex (MHC) class II DRB genotypes were examined in two geographically isolated populations of California sea lions (Zalophus californianus) (Gulf of California and California coastal Pacific Ocean). Genomic DNA from 227 California sea lions was examined using eight sequence-specific primer (SSP) pairs flanking the putative peptide-binding site. A total of 40 different Zaca-DRB genotype configurations were identified among the 227 individuals. Using SSP-PCR, significant differences were found between coastal California and Gulf of California Zalophus populations in numbers of DRB sequences per individual and configuration of sequences within individuals. Additionally, unique local patterns of MHC diversity were identified among the Midriff Island animals. These population differences are consistent with either ecologically distinct patterns of selection pressures and/or geographical isolation. The consequences of these partitioned MHC configurations at the population level are as yet unknown; however, the worldwide increase in emerging marine diseases lends urgency to their examination.

Original languageEnglish (US)
Pages (from-to)529-533
Number of pages5
JournalMolecular Ecology
Volume15
Issue number2
DOIs
StatePublished - Feb 2006

Fingerprint

Sea Lions
Dichlororibofuranosylbenzimidazole
major histocompatibility complex
pinniped
Major Histocompatibility Complex
Genes
gene
Gulf of California
genotype
Population
genes
isolated population
Genotype
Pacific Ocean
Zalophus
peptide
genomics
Animals
Binding Sites
Islands

Keywords

  • California sea lion
  • Class II MHC
  • DRB
  • Gulf of California
  • Pacific Ocean
  • Pathogen

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

MHC gene configuration variation in geographically disparate populations of California sea lions (Zalophus californianus). / Bowen, L.; Aldridge, B. M.; Delong, R.; Melin, S.; Godinez, C.; Zavala, A.; Gulland, F.; Lowenstine, Linda J; Stott, Jeffrey L; Johnson, M. L.

In: Molecular Ecology, Vol. 15, No. 2, 02.2006, p. 529-533.

Research output: Contribution to journalArticle

Bowen, L. ; Aldridge, B. M. ; Delong, R. ; Melin, S. ; Godinez, C. ; Zavala, A. ; Gulland, F. ; Lowenstine, Linda J ; Stott, Jeffrey L ; Johnson, M. L. / MHC gene configuration variation in geographically disparate populations of California sea lions (Zalophus californianus). In: Molecular Ecology. 2006 ; Vol. 15, No. 2. pp. 529-533.
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