MHC class II-expressing thymocytes suppress invariant NKT cell development

Wei Li, M. Hanief Sofi, Datsen G. Wei, Wenjun Du, Jacquelyn Gervay-Hague, Gourapura J. Renukaradhya, Randy R. Brutkiewicz, Cheong Hee Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Natural killer T (NKT) cells are positively selected on cortical thymocytes expressing the non-classical major histocompatibility complex (MHC) class I CD1d molecules. However, it is less clear how NKT cells are negatively selected in the thymus. In this study, we investigated the role of MHC class II expression in NKT cell development. Transgenic mice expressing MHC class II on thymocytes and peripheral T cells had a marked reduction in invariant NKT (iNKT) cells. Reduced numbers of iNKT cells correlated with the absence of in vivo production of cytokines in response to the iNKT cell agonist α-galactosylceramide. Using mixed bone marrow chimeras, we found that MHC class II-expressing thymocytes suppressed the development of iNKT cells in trans in a CD4-dependent manner. Our observations have significant implications for human iNKT cell development as human thymocytes express MHC class II, which can lead to an inefficient selection of iNKT cells.

Original languageEnglish (US)
Pages (from-to)186-189
Number of pages4
JournalImmunology and Cell Biology
Volume87
Issue number2
DOIs
StatePublished - Feb 2009

Fingerprint

Natural Killer T-Cells
Thymocytes
Major Histocompatibility Complex
CD1d Antigen
Galactosylceramides
Human Development
Thymus Gland
Transgenic Mice
Bone Marrow
Cytokines
T-Lymphocytes

Keywords

  • CD1d
  • iNKT cells
  • MHC class II
  • Thymocyte development
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Li, W., Sofi, M. H., Wei, D. G., Du, W., Gervay-Hague, J., Renukaradhya, G. J., ... Chang, C. H. (2009). MHC class II-expressing thymocytes suppress invariant NKT cell development. Immunology and Cell Biology, 87(2), 186-189. https://doi.org/10.1038/icb.2008.78

MHC class II-expressing thymocytes suppress invariant NKT cell development. / Li, Wei; Sofi, M. Hanief; Wei, Datsen G.; Du, Wenjun; Gervay-Hague, Jacquelyn; Renukaradhya, Gourapura J.; Brutkiewicz, Randy R.; Chang, Cheong Hee.

In: Immunology and Cell Biology, Vol. 87, No. 2, 02.2009, p. 186-189.

Research output: Contribution to journalArticle

Li, W, Sofi, MH, Wei, DG, Du, W, Gervay-Hague, J, Renukaradhya, GJ, Brutkiewicz, RR & Chang, CH 2009, 'MHC class II-expressing thymocytes suppress invariant NKT cell development', Immunology and Cell Biology, vol. 87, no. 2, pp. 186-189. https://doi.org/10.1038/icb.2008.78
Li, Wei ; Sofi, M. Hanief ; Wei, Datsen G. ; Du, Wenjun ; Gervay-Hague, Jacquelyn ; Renukaradhya, Gourapura J. ; Brutkiewicz, Randy R. ; Chang, Cheong Hee. / MHC class II-expressing thymocytes suppress invariant NKT cell development. In: Immunology and Cell Biology. 2009 ; Vol. 87, No. 2. pp. 186-189.
@article{09ba52fcbd004d69b898c4a1c2b597ec,
title = "MHC class II-expressing thymocytes suppress invariant NKT cell development",
abstract = "Natural killer T (NKT) cells are positively selected on cortical thymocytes expressing the non-classical major histocompatibility complex (MHC) class I CD1d molecules. However, it is less clear how NKT cells are negatively selected in the thymus. In this study, we investigated the role of MHC class II expression in NKT cell development. Transgenic mice expressing MHC class II on thymocytes and peripheral T cells had a marked reduction in invariant NKT (iNKT) cells. Reduced numbers of iNKT cells correlated with the absence of in vivo production of cytokines in response to the iNKT cell agonist α-galactosylceramide. Using mixed bone marrow chimeras, we found that MHC class II-expressing thymocytes suppressed the development of iNKT cells in trans in a CD4-dependent manner. Our observations have significant implications for human iNKT cell development as human thymocytes express MHC class II, which can lead to an inefficient selection of iNKT cells.",
keywords = "CD1d, iNKT cells, MHC class II, Thymocyte development, Transgenic mice",
author = "Wei Li and Sofi, {M. Hanief} and Wei, {Datsen G.} and Wenjun Du and Jacquelyn Gervay-Hague and Renukaradhya, {Gourapura J.} and Brutkiewicz, {Randy R.} and Chang, {Cheong Hee}",
year = "2009",
month = "2",
doi = "10.1038/icb.2008.78",
language = "English (US)",
volume = "87",
pages = "186--189",
journal = "Immunology and Cell Biology",
issn = "0818-9641",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - MHC class II-expressing thymocytes suppress invariant NKT cell development

AU - Li, Wei

AU - Sofi, M. Hanief

AU - Wei, Datsen G.

AU - Du, Wenjun

AU - Gervay-Hague, Jacquelyn

AU - Renukaradhya, Gourapura J.

AU - Brutkiewicz, Randy R.

AU - Chang, Cheong Hee

PY - 2009/2

Y1 - 2009/2

N2 - Natural killer T (NKT) cells are positively selected on cortical thymocytes expressing the non-classical major histocompatibility complex (MHC) class I CD1d molecules. However, it is less clear how NKT cells are negatively selected in the thymus. In this study, we investigated the role of MHC class II expression in NKT cell development. Transgenic mice expressing MHC class II on thymocytes and peripheral T cells had a marked reduction in invariant NKT (iNKT) cells. Reduced numbers of iNKT cells correlated with the absence of in vivo production of cytokines in response to the iNKT cell agonist α-galactosylceramide. Using mixed bone marrow chimeras, we found that MHC class II-expressing thymocytes suppressed the development of iNKT cells in trans in a CD4-dependent manner. Our observations have significant implications for human iNKT cell development as human thymocytes express MHC class II, which can lead to an inefficient selection of iNKT cells.

AB - Natural killer T (NKT) cells are positively selected on cortical thymocytes expressing the non-classical major histocompatibility complex (MHC) class I CD1d molecules. However, it is less clear how NKT cells are negatively selected in the thymus. In this study, we investigated the role of MHC class II expression in NKT cell development. Transgenic mice expressing MHC class II on thymocytes and peripheral T cells had a marked reduction in invariant NKT (iNKT) cells. Reduced numbers of iNKT cells correlated with the absence of in vivo production of cytokines in response to the iNKT cell agonist α-galactosylceramide. Using mixed bone marrow chimeras, we found that MHC class II-expressing thymocytes suppressed the development of iNKT cells in trans in a CD4-dependent manner. Our observations have significant implications for human iNKT cell development as human thymocytes express MHC class II, which can lead to an inefficient selection of iNKT cells.

KW - CD1d

KW - iNKT cells

KW - MHC class II

KW - Thymocyte development

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=60749129178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60749129178&partnerID=8YFLogxK

U2 - 10.1038/icb.2008.78

DO - 10.1038/icb.2008.78

M3 - Article

C2 - 18982019

AN - SCOPUS:60749129178

VL - 87

SP - 186

EP - 189

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

SN - 0818-9641

IS - 2

ER -