MHC class II association study in eight breeds of dog with hypoadrenocorticism

Jonathan Massey, Alisdair Boag, Andrea D. Short, Rachel A. Scholey, Paula S. Henthorn, Meryl P. Littman, Eystein Husebye, Brian Catchpole, Niels C Pedersen, Cathryn S. Mellersh, William E R Ollier, Lorna J. Kennedy

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Canine hypoadrenocorticism is an endocrine disorder characterised by inadequate secretion of steroid hormones from the adrenal glands. Pathology results from immune-mediated destruction of the adrenal cortex, which is similar to that seen in the human Addison's disease. Both the canine and human diseases have similar clinical presentation, with the diagnosis based on performing a dynamic adrenocorticotropic hormone stimulation test. MHC class II has previously been associated with the human and canine diseases. In the current study, we conducted an MHC class II association study in eight breeds of dog with diagnoses of hypoadrenocorticism. We demonstrated significant differences in dog leukocyte antigen (DLA) haplotype frequencies in six of these breeds: Cocker spaniel, Springer spaniel, Labrador, West Highland white terrier (WHWT), Bearded collie, and Standard poodle. In the Springer spaniel, the DLA-DRB1015:01 - DQA1006:01 - DQB1023:01 haplotype was significantly associated with disease risk (p = 0.014, odds ratio (OR) = 5.14) and showed a similar trend in the Cocker spaniel. This haplotype is related to one associated with hypoadrenocorticism in the Nova Scotia duck tolling retriever. Similar haplotypes shared between breeds were demonstrated, with DLA-DRB1001:01 - DQA1001:01 - DQB1002:01 more prevalent in both affected Labrador (p = 0.0002, OR = 3.06) and WHWT (p = 0.01, OR = 2.11). Other haplotypes that have not previously been associated with the disease were identified. The inter-breed differences in DLA haplotypes associated with susceptibility to canine hypoadrenocorticism could represent divergent aetiologies. This could have implications for clinical diagnosis and future comparative studies. Alternatively, it may suggest that the gene of interest is closely linked to the MHC.

Original languageEnglish (US)
Pages (from-to)291-297
Number of pages7
JournalImmunogenetics
Volume65
Issue number4
DOIs
StatePublished - Apr 2013

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Haplotypes
Dogs
HLA Antigens
Dog Diseases
Newfoundland and Labrador
Odds Ratio
Canidae
Nova Scotia
Addison Disease
Ducks
Adrenal Cortex
Adrenal Glands
Adrenocorticotropic Hormone
Steroids
Hormones
Pathology
Genes

Keywords

  • Addison's
  • DLA
  • Hypoadrenocorticism
  • MHC

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Massey, J., Boag, A., Short, A. D., Scholey, R. A., Henthorn, P. S., Littman, M. P., ... Kennedy, L. J. (2013). MHC class II association study in eight breeds of dog with hypoadrenocorticism. Immunogenetics, 65(4), 291-297. https://doi.org/10.1007/s00251-013-0680-2

MHC class II association study in eight breeds of dog with hypoadrenocorticism. / Massey, Jonathan; Boag, Alisdair; Short, Andrea D.; Scholey, Rachel A.; Henthorn, Paula S.; Littman, Meryl P.; Husebye, Eystein; Catchpole, Brian; Pedersen, Niels C; Mellersh, Cathryn S.; Ollier, William E R; Kennedy, Lorna J.

In: Immunogenetics, Vol. 65, No. 4, 04.2013, p. 291-297.

Research output: Contribution to journalArticle

Massey, J, Boag, A, Short, AD, Scholey, RA, Henthorn, PS, Littman, MP, Husebye, E, Catchpole, B, Pedersen, NC, Mellersh, CS, Ollier, WER & Kennedy, LJ 2013, 'MHC class II association study in eight breeds of dog with hypoadrenocorticism', Immunogenetics, vol. 65, no. 4, pp. 291-297. https://doi.org/10.1007/s00251-013-0680-2
Massey J, Boag A, Short AD, Scholey RA, Henthorn PS, Littman MP et al. MHC class II association study in eight breeds of dog with hypoadrenocorticism. Immunogenetics. 2013 Apr;65(4):291-297. https://doi.org/10.1007/s00251-013-0680-2
Massey, Jonathan ; Boag, Alisdair ; Short, Andrea D. ; Scholey, Rachel A. ; Henthorn, Paula S. ; Littman, Meryl P. ; Husebye, Eystein ; Catchpole, Brian ; Pedersen, Niels C ; Mellersh, Cathryn S. ; Ollier, William E R ; Kennedy, Lorna J. / MHC class II association study in eight breeds of dog with hypoadrenocorticism. In: Immunogenetics. 2013 ; Vol. 65, No. 4. pp. 291-297.
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