Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA

Shelly Meeusen, Quinton Tieu, Edith Wong, Eric Weiss, David Schieltz, John R. Yates, Jodi Nunnari

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Maintenance of mitochondrial DNA (mtDNA) during cell division is required for progeny to be respiratory competent. Maintenance involves the replication, repair, assembly, segregation, and partitioning of the mitochondrial nucleoid. MGM101 has been identified as a gene essential for mtDNA maintenance in S. cerevisiae, but its role is unknown. Using liquid chromatography coupled with tandem mass spectrometry, we identified Mgm101p as a component of highly enriched nucleoids, suggesting that it plays a nucleoid-specific role in maintenance. Subcellular fractionation, indirect immunofluorescence and GFP tagging show that Mgm101p is exclusively associated with the mitochondrial nucleoid structure in cells. Furthermore, DNA affinity chromatography of nucleoid extracts indicates that Mgm101p binds to DNA, suggesting that its nucleoid localization is in part due to this activity. Phenotypic analysis of cells containing a temperature sensitive mgm101 allele suggests that Mgm101p is not involved in mtDNA packaging, segregation, partitioning or required for ongoing mtDNA replication. We examined Mgm101p's role in mtDNA repair. As compared with wild-type cells, mgm101 cells were more sensitive to mtDNA damage induced by UV irradiation and were hypersensitive to mtDNA damage induced by gamma rays and H2O2 treatment. Thus, we propose that Mgm101p performs an essential function in the repair of oxidatively damaged mtDNA that is required for the maintenance of the mitochondrial genome.

Original languageEnglish (US)
Pages (from-to)291-304
Number of pages14
JournalJournal of Cell Biology
Volume145
Issue number2
DOIs
StatePublished - Jun 2000

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Mitochondrial DNA
DNA
Maintenance
DNA Damage
DNA Packaging
Mitochondrial Genome
Gamma Rays
Essential Genes
Indirect Fluorescent Antibody Technique
Tandem Mass Spectrometry
DNA Replication
Affinity Chromatography
Liquid Chromatography
DNA Repair
Cell Division
Saccharomyces cerevisiae
Alleles
Temperature

Keywords

  • DNA repair
  • Maintenance
  • MtDNA
  • Nucleoid
  • Yeast

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA. / Meeusen, Shelly; Tieu, Quinton; Wong, Edith; Weiss, Eric; Schieltz, David; Yates, John R.; Nunnari, Jodi.

In: Journal of Cell Biology, Vol. 145, No. 2, 06.2000, p. 291-304.

Research output: Contribution to journalArticle

Meeusen, Shelly ; Tieu, Quinton ; Wong, Edith ; Weiss, Eric ; Schieltz, David ; Yates, John R. ; Nunnari, Jodi. / Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA. In: Journal of Cell Biology. 2000 ; Vol. 145, No. 2. pp. 291-304.
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