The development of a microassay for proteinase K esterase activity with carboxylic acid esters is reported using novel substrates of the general formula R-C(O)-XR′. Highest rates of hydrolysis have been obtained with the O-phenyl esters CH3(CH2)n = 1-2-S-(CH2)n=1-2C(O)-O-phenyl and their thioester analogs in studies where R, X and R′ have been varied. The phenol release has been measured with 4-aminoantipyrine and potassium ferricyanide to determine the rates of O-phenyl ester hydrolyses. Thioester hydrolyses have been monitored continuously with 5,5′-dithio-bis (2-nitrobenzoic acid).
|Original language||English (US)|
|Number of pages||3|
|Journal||Fresenius' Journal of Analytical Chemistry|
|State||Published - Feb 2 1996|
ASJC Scopus subject areas
- Clinical Biochemistry
- Analytical Chemistry