Methods to obtain radiolabelled monocrotaline

M. W. Lamé, D. Morin, Dennis W Wilson, H. J. Segall

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Crotalaria spectabilis, a plant found in many areas of the world is associated with the pyrrolizidine alkaloid monocrotaline. Monocrotaline when injected subcutaneously in Sprague Dawley rats has been utilized for years to create a condition known to mimic pulmonary hypertension in humans. We attempted to determine the optimum conditions for the biosynthesis of radiolabelled monocrotaline. Our work describes the plant growth conditions and the time periods associated with the production of radiolabelled monocrotaline. In addition, the incorporation of 14CO2 or [2,3-3H]-putrescine dihydrochloride and the specific activity plus the amount(s) of recovered radiolabelled monocrotaline are discussed. We conclude that the most efficient and cost effective method for the biosynthesis of radiolabelled monocrotaline is still the utilization of 14CO2.

Original languageEnglish (US)
Pages (from-to)1053-1060
Number of pages8
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume38
Issue number12
DOIs
StatePublished - Dec 1996

Keywords

  • Biosynthesis
  • Crotalaria
  • Monocrotaline
  • Putrescine
  • Pyrrolizidine
  • Radiolabelled

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

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