Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-12
Number of pages12
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1458
ISSN (Print)1064-3745

Fingerprint

Fluorescent Antibody Technique
Immunohistochemistry
Cellular Microenvironment
Equipment and Supplies

Keywords

  • Autofluorescence
  • Formalin-fixed paraffinembedded tissues
  • Immunofluorescence
  • Immunohistochemistry
  • Zinc fixation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mori, H., & Cardiff, R. (2016). Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible. In Methods in Molecular Biology (pp. 1-12). (Methods in Molecular Biology; Vol. 1458). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3801-8_1

Methods of immunohistochemistry and immunofluorescence : Converting invisible to visible. / Mori, Hidetoshi; Cardiff, Robert.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 1-12 (Methods in Molecular Biology; Vol. 1458).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mori, H & Cardiff, R 2016, Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1458, Humana Press Inc., pp. 1-12. https://doi.org/10.1007/978-1-4939-3801-8_1
Mori H, Cardiff R. Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 1-12. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3801-8_1
Mori, Hidetoshi ; Cardiff, Robert. / Methods of immunohistochemistry and immunofluorescence : Converting invisible to visible. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 1-12 (Methods in Molecular Biology).
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