Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-12
Number of pages12
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1458
ISSN (Print)1064-3745

Keywords

  • Autofluorescence
  • Formalin-fixed paraffinembedded tissues
  • Immunofluorescence
  • Immunohistochemistry
  • Zinc fixation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mori, H., & Cardiff, R. (2016). Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible. In Methods in Molecular Biology (pp. 1-12). (Methods in Molecular Biology; Vol. 1458). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3801-8_1