Abstract
Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 1-12 |
| Number of pages | 12 |
| DOIs | |
| State | Published - Jan 1 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1458 |
| ISSN (Print) | 1064-3745 |
Keywords
- Autofluorescence
- Formalin-fixed paraffinembedded tissues
- Immunofluorescence
- Immunohistochemistry
- Zinc fixation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Mori, H., & Cardiff, R. (2016). Methods of immunohistochemistry and immunofluorescence: Converting invisible to visible. In Methods in Molecular Biology (pp. 1-12). (Methods in Molecular Biology; Vol. 1458). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3801-8_1