Abstract
Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 1-12 |
| Number of pages | 12 |
| DOIs | |
| State | Published - Jan 1 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1458 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Autofluorescence
- Formalin-fixed paraffinembedded tissues
- Immunofluorescence
- Immunohistochemistry
- Zinc fixation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Methods of immunohistochemistry and immunofluorescence : Converting invisible to visible. / Mori, Hidetoshi; Cardiff, Robert.
Methods in Molecular Biology. Humana Press Inc., 2016. p. 1-12 (Methods in Molecular Biology; Vol. 1458).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Methods of immunohistochemistry and immunofluorescence
T2 - Converting invisible to visible
AU - Mori, Hidetoshi
AU - Cardiff, Robert
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.
AB - Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the “pros and cons” in each method, along with alternative procedures and the suggested imaging equipment.
KW - Autofluorescence
KW - Formalin-fixed paraffinembedded tissues
KW - Immunofluorescence
KW - Immunohistochemistry
KW - Zinc fixation
UR - http://www.scopus.com/inward/record.url?scp=85007433713&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007433713&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3801-8_1
DO - 10.1007/978-1-4939-3801-8_1
M3 - Chapter
C2 - 27581010
AN - SCOPUS:85007433713
T3 - Methods in Molecular Biology
SP - 1
EP - 12
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -