Methods for studying the vector competence of Culex tarsalis for western equine encephalomyelitis virus

Farida Mahmood, Robert E. Chiles, Ying Fang, William Reisen

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Female Culex tarsalis fed heparinized chicken blood-western equine encephalomyelitis virus (WEEV) mixtures through a biomembrane feeder were compared with females fed sweetened blood-virus mixtures presented in pledgets or as hanging drops or to restrained chickens with natural or artificial viremias. Results indicated that sodium heparin did not adversely affect the infection of Culex tarsalis with WEEV. Overall advantages of the biomembrane system included 1) increased blood feeding frequency, 2) control of the infectious virus dose, and 3) greater or similar infection rates and body titers to females taking blood meals from viremic chickens. Anesthetizing females with triethylamine for in vitro transmission assessment using the capillary tube method produced results similar to immobilization using cold or CO2 + cold. Our research provided insight into tools useful to investigate the infection and transmission of WEEV by Cx. tarsalis.

Original languageEnglish (US)
Pages (from-to)277-282
Number of pages6
JournalJournal of the American Mosquito Control Association
Volume20
Issue number3
StatePublished - Sep 1 2004

Keywords

  • Culex tarsalis
  • Heparin
  • Membrane feeder
  • Triethylamine
  • Vector competence
  • Western equine encephalomyelitis virus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Public Health, Environmental and Occupational Health
  • Insect Science

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