Methods for introducing morpholinos into the chicken embryo

Robert Kos, Richard P Tucker, Ronelle Hall, Tuan D. Duong, Carol A. Erickson

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The use of antisense morpholino oligos to inhibit the translation of a target transcript has been applied recently to studies of the chicken embryo. In contrast to other developmental systems such as in frog, sea urchin, and zebrafish that permit the direct microinjection of morpholinos into a blastomere, square pulse electroporation is used to introduce fluorescently tagged morpholinos into specific populations of chick embryo cells in ovo. This article reviews the methods that have proven successful, the types of controls that are necessary when performing knockdowns of gene expression in the chick embryo, and discusses the limitations of the current technique, as well as directions for further research.

Original languageEnglish (US)
Pages (from-to)470-477
Number of pages8
JournalDevelopmental Dynamics
Volume226
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

Morpholinos
Chickens
Embryonic Structures
Chick Embryo
Blastomeres
Electroporation
Sea Urchins
Microinjections
Zebrafish
Anura
Gene Expression
Research
Population

Keywords

  • Chick embryos
  • Delivery methods
  • Morpholinos

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Methods for introducing morpholinos into the chicken embryo. / Kos, Robert; Tucker, Richard P; Hall, Ronelle; Duong, Tuan D.; Erickson, Carol A.

In: Developmental Dynamics, Vol. 226, No. 3, 01.03.2003, p. 470-477.

Research output: Contribution to journalArticle

Kos, Robert ; Tucker, Richard P ; Hall, Ronelle ; Duong, Tuan D. ; Erickson, Carol A. / Methods for introducing morpholinos into the chicken embryo. In: Developmental Dynamics. 2003 ; Vol. 226, No. 3. pp. 470-477.
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