Methods and applications of Raman microspectroscopy to single-cell analysis

Iwan W. Schie, Thomas R Huser

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Raman spectroscopy is a powerful biochemical analysis technique that allows for the dynamic characterization and imaging of living biological cells in the absence of fluorescent stains. In this review, we summarize some of the most recent developments in the noninvasive biochemical characterization of single cells by spontaneous Raman scattering. Different instrumentation strategies utilizing confocal detection optics, multispot, and line illumination have been developed to improve the speed and sensitivity of the analysis of single cells by Raman spectroscopy. To analyze and visualize the large data sets obtained during such experiments, sophisticated multivariate statistical analysis tools are necessary to reduce the data and extract components of interest. We highlight the most recent applications of single cell analysis by Raman spectroscopy and their biomedical implications that have enabled the noninvasive characterization of specific metabolic states of eukaryotic cells, the identification and characterization of stem cells, and the rapid identification of bacterial cells. We conclude the article with a brief look into the future of this rapidly evolving research area.

Original languageEnglish (US)
Pages (from-to)813-828
Number of pages16
JournalApplied Spectroscopy
Volume67
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Raman spectroscopy
cells
multivariate statistical analysis
Stem cells
stem cells
Raman scattering
Optics
Statistical methods
Coloring Agents
Lighting
Imaging techniques
illumination
optics
Raman spectra
sensitivity
Experiments

Keywords

  • Intralipid
  • Inverse adding-doubling
  • Optical microscopy
  • Optical properties
  • Optical spectroscopy
  • Raman scattering
  • Temperature stability

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Methods and applications of Raman microspectroscopy to single-cell analysis. / Schie, Iwan W.; Huser, Thomas R.

In: Applied Spectroscopy, Vol. 67, No. 8, 08.2013, p. 813-828.

Research output: Contribution to journalArticle

Schie, Iwan W. ; Huser, Thomas R. / Methods and applications of Raman microspectroscopy to single-cell analysis. In: Applied Spectroscopy. 2013 ; Vol. 67, No. 8. pp. 813-828.
@article{7289fda726de478e9b10100429ce9959,
title = "Methods and applications of Raman microspectroscopy to single-cell analysis",
abstract = "Raman spectroscopy is a powerful biochemical analysis technique that allows for the dynamic characterization and imaging of living biological cells in the absence of fluorescent stains. In this review, we summarize some of the most recent developments in the noninvasive biochemical characterization of single cells by spontaneous Raman scattering. Different instrumentation strategies utilizing confocal detection optics, multispot, and line illumination have been developed to improve the speed and sensitivity of the analysis of single cells by Raman spectroscopy. To analyze and visualize the large data sets obtained during such experiments, sophisticated multivariate statistical analysis tools are necessary to reduce the data and extract components of interest. We highlight the most recent applications of single cell analysis by Raman spectroscopy and their biomedical implications that have enabled the noninvasive characterization of specific metabolic states of eukaryotic cells, the identification and characterization of stem cells, and the rapid identification of bacterial cells. We conclude the article with a brief look into the future of this rapidly evolving research area.",
keywords = "Intralipid, Inverse adding-doubling, Optical microscopy, Optical properties, Optical spectroscopy, Raman scattering, Temperature stability",
author = "Schie, {Iwan W.} and Huser, {Thomas R}",
year = "2013",
month = "8",
doi = "10.1366/12-06971",
language = "English (US)",
volume = "67",
pages = "813--828",
journal = "Applied Spectroscopy",
issn = "0003-7028",
publisher = "Society for Applied Spectroscopy",
number = "8",

}

TY - JOUR

T1 - Methods and applications of Raman microspectroscopy to single-cell analysis

AU - Schie, Iwan W.

AU - Huser, Thomas R

PY - 2013/8

Y1 - 2013/8

N2 - Raman spectroscopy is a powerful biochemical analysis technique that allows for the dynamic characterization and imaging of living biological cells in the absence of fluorescent stains. In this review, we summarize some of the most recent developments in the noninvasive biochemical characterization of single cells by spontaneous Raman scattering. Different instrumentation strategies utilizing confocal detection optics, multispot, and line illumination have been developed to improve the speed and sensitivity of the analysis of single cells by Raman spectroscopy. To analyze and visualize the large data sets obtained during such experiments, sophisticated multivariate statistical analysis tools are necessary to reduce the data and extract components of interest. We highlight the most recent applications of single cell analysis by Raman spectroscopy and their biomedical implications that have enabled the noninvasive characterization of specific metabolic states of eukaryotic cells, the identification and characterization of stem cells, and the rapid identification of bacterial cells. We conclude the article with a brief look into the future of this rapidly evolving research area.

AB - Raman spectroscopy is a powerful biochemical analysis technique that allows for the dynamic characterization and imaging of living biological cells in the absence of fluorescent stains. In this review, we summarize some of the most recent developments in the noninvasive biochemical characterization of single cells by spontaneous Raman scattering. Different instrumentation strategies utilizing confocal detection optics, multispot, and line illumination have been developed to improve the speed and sensitivity of the analysis of single cells by Raman spectroscopy. To analyze and visualize the large data sets obtained during such experiments, sophisticated multivariate statistical analysis tools are necessary to reduce the data and extract components of interest. We highlight the most recent applications of single cell analysis by Raman spectroscopy and their biomedical implications that have enabled the noninvasive characterization of specific metabolic states of eukaryotic cells, the identification and characterization of stem cells, and the rapid identification of bacterial cells. We conclude the article with a brief look into the future of this rapidly evolving research area.

KW - Intralipid

KW - Inverse adding-doubling

KW - Optical microscopy

KW - Optical properties

KW - Optical spectroscopy

KW - Raman scattering

KW - Temperature stability

UR - http://www.scopus.com/inward/record.url?scp=84882338791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882338791&partnerID=8YFLogxK

U2 - 10.1366/12-06971

DO - 10.1366/12-06971

M3 - Article

VL - 67

SP - 813

EP - 828

JO - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 0003-7028

IS - 8

ER -