Method for the detection of desmethylbromethalin in animal tissue samples for the determination of bromethalin exposure

Michael S. Filigenzi, Adrienne C. Bautista, Linda S. Aston, Robert H Poppenga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bromethalin, a potent neurotoxin, is widely available for use as a rodenticide. As access to other rodenticides is reduced due to regulatory pressure, the use of bromethalin is likely to increase with a concomitant increase in poisonings in nontarget animals. Analytical methods for the detection of bromethalin residues in animals suspected to have been exposed to this rodenticide are needed to support post-mortem diagnosis of toxicosis. This paper describes a novel method for the analysis of desmethylbromethalin (DMB), bromethalin's toxic metabolite, in tissue samples such as liver, brain, and adipose. Samples were extracted with 5% ethanol in ethyl acetate, and an aliquot of the extract was evaporated dry, reconstituted, and analyzed by reverse phase ultrahigh-performance liquid chromatography-mass spectrometry. The mass spectrometer utilized electrospray ionization in negative ion mode with multiple reaction monitoring. This method was qualitatively validated at a level of 1.0 ng/g in liver tissue. The quantitative potential of the method was also evaluated, and a method detection limit of 0.35 ng/g wet weight was determined in fat tissue. DMB was detected in tissue samples from animals suspected to have been poisoned by this compound. To the authors' knowledge, there have been no other methods reported for analysis of DMB in tissue samples using LC-MS/MS.

Original languageEnglish (US)
Pages (from-to)5146-5151
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number21
DOIs
StatePublished - Jun 3 2015

Fingerprint

animal tissues
Rodenticides
Animals
rodenticides
Tissue
Liver
poisoning
sampling
ultra-performance liquid chromatography
Electrospray ionization
methodology
animals
tissue analysis
liver
neurotoxins
reversed-phase liquid chromatography
Poisons
Liquid chromatography
Neurotoxins
Mass spectrometers

Keywords

  • bromethalin
  • desmethylbromethalin
  • electrospray
  • LC-MS/MS
  • poisoning

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Method for the detection of desmethylbromethalin in animal tissue samples for the determination of bromethalin exposure. / Filigenzi, Michael S.; Bautista, Adrienne C.; Aston, Linda S.; Poppenga, Robert H.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 21, 03.06.2015, p. 5146-5151.

Research output: Contribution to journalArticle

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